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New Delhi metallo-β-lactamase (NDM-1) is a new metallo-β-lactamase (MBL) that has recently emerged as a global threat because it confers bacteria with resistance to almost all clinically used β-lactam antibiotics. To determine the molecular basis of this threat, NDM-1 was purified from Escherichia coli TransB (DE3) carrying cloned blaNDM-1 gene by an anion-exchange chromatography step followed ...
Antibiotic resistance is a public health problem. The emergence of carbapenemase-producing Enterobacterales (CPE) infections concern, particularly in Senegal. (1) Methods: Between January 2019 and July 2022, 240 isolates enterobacteria resistant to third-generation cephalosporins imipenem from biological samples Fann Hospital (Dakar) Hôpital Paix (Ziguinchor) were selected. identified by MALDI-...
Detection of New Delhi Metallo-β-Lactamase (blaNDM-1) in Acinetobacter schindleri during 1 routine surveillance 2 Patrick McGann, Michael Milillo, Robert J. Clifford, Erik Snesrud, Lindsay Stevenson, 3 Michael G. Backlund, Helen B. Viscount, Reyes Quintero, Yoon I Kwak, Michael J. Zapor, 4 Paige E Waterman, Emil P Lesho 5 1 Multidrug-resistant Organism Repository and Surveillance Network, Walte...
BACKGROUND The gene for New Delhi metallo-β-lactamase 1 (NDM-1) has been reported to be transmitted via plasmids which are easily transferable and capable of wide distribution. We report the isolation of two NDM-1 producing strains and possible in vivo transfer of blaNDM-1 in a patient. METHODS Clinical samples were collected for bacterial culture and antibiotic susceptibility testing from a ...
Sir, Recently, a novel New Delhi metallo-b-lactamase (NDM) variant was described by Nordmann et al. This variant, designated NDM-4, differs from NDM-1 by a single amino substitution and an increased carbapenemase activity. NDM-4 was first detected in an Escherichia coli isolated from a urinary sample from a patient previously hospitalized in India. A second finding of the NDM-4 variant was repo...
Objective(s): Resistance to carbapenems is the principal reason for the continuing utilization of colistin as a last resort choice for treating the infections resulted from multidrug carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates. The assessment of antimicrobial resistance pattern, the prevalence of carbapenem-resistance determinants, and molecular epidemiology of colistin-resistan...
OBJECTIVE To review the origin, diagnosis, treatment and public health concern of New Delhi metallo-β-lactamase (NDM)-producing bacteria. DATA SOURCES We searched database for studies published in English. The database of PubMed from 2007 to 2015 was used to conduct a search using the keyword term "NDM and Acinetobacter or Enterobacteriaceae or Pseudomonas aeruginosa." STUDY SELECTION We co...
Bacteria that produce the broad-spectrum Carbapenem antibiotic New Delhi Metallo-β-lactamase (NDM) place a burden on health care systems worldwide, due to the limited treatment options for infections caused by them and the rapid global spread of this antibiotic resistance mechanism. Although it is believed that the associated resistance gene blaNDM-1 originated in Acinetobacter spp., the role o...
Since the first report of blaNDM-1, 16 blaNDM variants have been identified among Gram-negative bacteria worldwide. Recently, a novel blaNDM variant, blaNDM-13, was identified in the chromosome of an ST101 Escherichia coli isolate from Nepal. Here we first reported plasmid-mediated blaNDM-13 in a carbapenem-resistant E. coli ST5138 clinical isolate associated with hospital-acquired urinary trac...
Pseudomonas aeruginosa (P. aeruginosa) places among major opportunistic nosocomial pathogen which has developed extensive drug resistance. Due to uncontrolled consumption of antibiotics, multidrug-resistant (MDR) P. species are increasingly isolated from various settings hospitals globally. This research aimed determine the genes reproducing blaOXA-48, blaIMP-1, blaVIM-1 and blaNDM-1 metallo be...
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