We have developed an automated enzymatic assay for LDH-C4, a lactate dehydrogenase (EC 1.1.1.27) isoenzyme found exclusively in spermatozoa, as a marker of human spermatogenesis. LDH-C4 activity is assayed bichromatically in the Abbott ABA-100 spectrophotometer, with 2-oxohexanoate as substrate. As many as 27 samples (2.5 microL) of human seminal plasma can be analyzed sequentially, with appare...

the aim of this study was to compare the effect of six weeks of volleyball specific training on the sand and hard surface (sport hall) on the resting levels and responses of lactate, creatine kinase (ck) and lactate dehydrogenase (ldh) to acute exercise in volleyball players. for this purpose, 18 male volleyball players (mean±sd; age, 23.7±3.7; weight, 75.3± 1.7) were randomly assigned to two g...

The cerebrospinal fluid concentration of Glutamine and Lactate dehydrogenase (LDH) were studied in patients with pyogenic and tubercular meningitis. Significant increase in Glutamine and LDH level (P<0.001) were observed in the test group when compared to the control group. LDH and glutamine may not be useful in differentiating viral from other meningitis. It may act as corroborative evidence o...

Lactate dehydrogenase A (LDH-A), which regulates glycolytic flux by catalyzing pyruvate to lactate in the cytoplasm, is believed to be one of the highly attractive therapeutic targets for cancers. Firstly, we detected the expression of LDH-A in gastric cancer (GC) cells. LDH-A inhibitor oxamate was then used to suppress the LDH-A activity in GC cells. Cell proliferation, lactic acid production,...

Lactate dehydrogenase (LDH) is a ubiquitous enzyme, which has the pivotal role in the clinical diagnosis of many disease processes. LDH is known to catalyze the oxidative conversion of the substrate pyruvate to lactate and has been used as an infl ammatory marker. LDH is routinely present in the cytoplasm of the cell that gets released into the extracellular environment upon cellular lysis and ...

Abstract Objective Removing plasma from the platelet concentrate (PC) medium could be an effective way to increase the safety of this product. The goal of this study was to compare PC stored in plasma or in an additive solution (Composol) with in vitro testing. Materials and Methods Fifty-four single donor PCs were prepared from Iranian Blood Transfusion Organization (IBTO). Each PC unit wa...

Streptococcus pneumoniae is a fermentative microorganism and causes serious diseases in humans, including otitis media, bacteremia, meningitis, and pneumonia. However, the mechanisms enabling pneumococcal survival in the host and causing disease in different tissues are incompletely understood. The available evidence indicates a strong link between the central metabolism and pneumococcal virule...

Fluorescence spectroscopy was used to study some aspects of thermal denaturation of lactate dehydrogenase (LDH) from rabbit muscle, in the absence and presence of urea and NaCl. Thermal denaturation of LDH seems to be one step and a slightly reversible process. The solution pH has an important influence on the LDH thermal denaturation. The interaction of LDH enzyme with NADH ligand was also inv...

Activities for nicotinamide adenine dinucleotide (NAD)-dependent and NAD-independent forms of lactate dehydrogenase (LDH) were measured in cell-free extracts of Staphylococcus aureus strain PS 6 for the d and l isomers of lactate. Data obtained for the NAD-dependent lactate dehydrogenases indicate that oxidation of both isomers of lactate is due to both an l-lactate-specific LDH and a lactate r...

The electrochemical entrapment of polyaniline (PANI) onto sol-gel derived tetraethylorthosilicate (TEOS) films deposited onto indium-tin-oxide (ITO) coated glass has been utilized for immobilization of lactate dehydrogenase (LDH). The performance of these sol-gel/PANI/LDH electrodes has been investigated as a function of the lactate concentration, applied potential, pH of the medium and interfe...