The formation of fibrocartilage during articular cartilage regeneration remains a clinical problem affecting adequate restoration in joints. To stimulate chondrocytes to form cartilage, we investigated the use amyloid fibril-based scaffolds. proteins α-synuclein, β-lactoglobulin, and lysozyme were induced self-assemble into fibrils and, dialysis, formed micrometer scale networks that resemble e...

It has already been reported that the principal protein of bovine colostrum is immune lactoglobulin, and a procedure has been described for the isolation of this protein (1). While the immune properties of colostrum have been generally recognized, it is widely believed that this is a specific quality of colostrum, and that immune properties are absent from the later milk or are present only in ...

• The gelatin and ?-lactoglobulin are adsorbed on TiO 2 nanoparticles. proteins improve the colloidal stability of prevent salt-induced aggregation enhance penetration inside human intestinal epithelial cells. favored most efficiently nanoparticles cell penetration. known as E171 one controversial food additive due to its potential toxicity. In this work, main hypothesis is that their favor To ...

Binding studies of cationic detergents such as cetyltrimethyl ammonium bromide, Cetyl pyridinium bromide and dodecyl trimethyl ammonium bromide with β Lactoglobulin were carried out by equilibrium dialysis, ultraviolet difference and circular dichroism techniques at 25 C. Binding isotherms at pH 5·0, 7·0 and 9·0 show cooperative binding at all concentrations of detergents and the number of avai...

Characterization of all gas-phase charge sites of natively sprayed proteins and peptides is demonstrated using 193 nm UVPD. The high sequence coverage offered by UVPD is exploited for the accurate determination of charge sites in protein systems up to 18 kDa, allowing charge site to be studied as a function of protein conformation and the presence of disulfide bonds. Charging protons are found ...

Functional recombinant bovine β-lactoglobulin has been produced by expression in E. coli using an engineered protein gene and purified to homogeneity by applying a new protocol. Mutations L1A/I2S introduced into the protein sequence greatly facilitate in vivo cleavage of the N-terminal methionine, allowing correctly folded and soluble protein suitable for biochemical, biophysical and structural...

The gene encoding human tear prealbumin, a major component of the protein fraction of tear fluid, was cloned from total cDNA of lacrimal gland by polymerase chain reaction using synthetic oligonucleotides derived from N-terminal amino acid sequences of the purified protein. Sequence analysis and a computer-assisted homology search revealed this protein to be a member of the lipocalin superfamil...

the objective of this study was to determine the polymorphism of bovine β-lactoglobulin (β-lg) gene and its association with milk production traits in iranian najdi cattle. blood samples were collected from 80 najdi cattle from the najdi cattle breeding station located in shoshtar, khuzestan. polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) techniqus were used...

Maximum likelihood methodology was used to estimate effects of both a marker gene and a linked quantitative trait locus (QTL) on quantitative traits in a segregating population. Two alleles were assumed for the QTL. In addition to the effects of genotypes at both loci on the mean of the quantitative trait, recombination frequency between the loci, frequency of the QTL alleles and the residual s...

Biopolymer nanoparticles can be formed by heating globular protein/polysaccharide mixtures above the thermal denaturation temperature of the protein under pH conditions where the two biopolymers are weakly electrically attracted to each other. In this study, the influence of polysaccharide linear charge density on the formation and properties of these biopolymer nanoparticles was examined. Mixe...