We perform Atomic Force Microscopy and numerical simulations of a bimodal solution containing long, semiflexible β-lactoglobulin fibrils and short, flexible β-lactoglobulin linear aggregates at an air-water interface. Short aggregates orient perpendicular to fibrils at very short distances and preferentially parallel at intermediate distances. At even larger distances an isotropic distribution ...

the genotypes for beta-lactoglobulin (blg) and calpastatin (cast) were determined by polymerase chain reaction (pcr) and restriction enzyme digestion and genotyped for calpain (capn) by pcr-sscp method in a native iranian breed sheep, kordi. blood samples were collected from 100 pure bred kordi sheep from kordi breeding station located in shirvan, mashhad. the extraction of genomic dna was base...

We introduce a green and efficient approach for removing per- polyfluoroalkyl substances (PFASs) based on the ?-lactoglobulin amyloid fibril membrane.

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The formation of fibrocartilage during articular cartilage regeneration remains a clinical problem affecting adequate restoration in joints. To stimulate chondrocytes to form cartilage, we investigated the use amyloid fibril-based scaffolds. proteins α-synuclein, β-lactoglobulin, and lysozyme were induced self-assemble into fibrils and, dialysis, formed micrometer scale networks that resemble e...

It has already been reported that the principal protein of bovine colostrum is immune lactoglobulin, and a procedure has been described for the isolation of this protein (1). While the immune properties of colostrum have been generally recognized, it is widely believed that this is a specific quality of colostrum, and that immune properties are absent from the later milk or are present only in ...

• The gelatin and ?-lactoglobulin are adsorbed on TiO 2 nanoparticles. proteins improve the colloidal stability of prevent salt-induced aggregation enhance penetration inside human intestinal epithelial cells. favored most efficiently nanoparticles cell penetration. known as E171 one controversial food additive due to its potential toxicity. In this work, main hypothesis is that their favor To ...

Binding studies of cationic detergents such as cetyltrimethyl ammonium bromide, Cetyl pyridinium bromide and dodecyl trimethyl ammonium bromide with β Lactoglobulin were carried out by equilibrium dialysis, ultraviolet difference and circular dichroism techniques at 25 C. Binding isotherms at pH 5·0, 7·0 and 9·0 show cooperative binding at all concentrations of detergents and the number of avai...

Characterization of all gas-phase charge sites of natively sprayed proteins and peptides is demonstrated using 193 nm UVPD. The high sequence coverage offered by UVPD is exploited for the accurate determination of charge sites in protein systems up to 18 kDa, allowing charge site to be studied as a function of protein conformation and the presence of disulfide bonds. Charging protons are found ...

Functional recombinant bovine β-lactoglobulin has been produced by expression in E. coli using an engineered protein gene and purified to homogeneity by applying a new protocol. Mutations L1A/I2S introduced into the protein sequence greatly facilitate in vivo cleavage of the N-terminal methionine, allowing correctly folded and soluble protein suitable for biochemical, biophysical and structural...