OBJECTIVE To assess the effect of implementing different previtrification interventions on the postwarming DNA integrity of vitrified blastocysts. DESIGN Prospective in vitro study. SETTING Center for Reproductive Medicine laboratory in a tertiary hospital setting. ANIMALS A total of 70 expanded and 46 nonexpanded mouse blastocysts were used for the study. INTERVENTION(S) [1] Twenty-two...

BACKGROUND It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers. METHODS In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the z...

BACKGROUND Routine use of assisted hatching (AH) following ICSI is a controversial issue in the literature. There are rare studies regarding the effect of laser assisted hatching (LAH) on live birth rate. OBJECTIVE Our main goal was to evaluate the effect of LAH on delivery rate as well as congenital anomaly in patients undergoing their first ICSI cycle. MATERIALS AND METHODS A total of 182...

introduction: implant bed preparation with laser is taken into consideration owing to the increased interest in use of lasers in hard tissue surgery. the purpose of this study is to determine the deviations in the position and inclination between the planned and prepared implant beds with erbium, chromium doped yttrium scandium gallium garnet (er,cr: ysgg) laser using stereolithographic (sla) s...

Objective This study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers. Methods First, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinni...