Bennett RD and Heftmann E (1962) Thin-layer chromatography of sterols. Journal of Chromatography 9: 359. Copius-Peereboom JW and Beekes HW (1965) The analysis of mixtures of animal and vegetable fats. V. Separation of sterol acetates by thin-layer chromatography in reversed-phase systems and on silica gel G} silver nitrate layers. Journal of Chromatography 17: 99. Hung GWC and Harris AZ (1989) ...

Ceramides with mono-, di-, and trihydroxy long-chain bases, and normal (saturated and unsaturated), branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography. In most cases the compounds were also run as acetates. Borate, arsenite, and silver ions were used as complexing agents, and the effects of number, position, and stereochemistry of hydroxy groups, and of un...

The various OPLC andRPC techniques are compared in Table 1. Study of the data shows that OPLC is an excellent technique for analytical separations and that RPC is more ideally suited as a preparative method for isolation of compounds from biological matrices. The advantage of combining online and ofSine separations and two-dimensional development can also be exploited in OPLC. The advantage of ...

There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is...