Inorganic lead ion in micromolar concentrations inhibits Electrophorus electroplax microsomal (Na+ + K+)-adenosine triphosphatase ((Na+ + K+)-ATPase) and K+-p-nitrophenylphosphatase (NPPase). Under the same conditions, the same concentrations of PbCl, that inhibit ATPase activity also stimulate the phosphorylation of electroplax microsomes in the absence of added Na+. Enzyme activity is protect...

A miniaturized lead sensor has been developed by combining a lead-specific DNAzyme with a microfabricated device containing a network of microfluidic channels that are fluidically coupled via a nanocapillary array interconnect. A DNAzyme construct, selective for cleavage in the presence of Pb2+ and derivatized with fluorophore (quencher) at the 5' (3') end of the substrate and enzyme strands, r...

The ribonucleoprotein (RNP) complex is the essential transcription-replication machinery of the influenza virus. It is composed of the trimeric polymerase (PA, PB1 and PB2), nucleoprotein (NP) and RNA. Elucidating the molecular mechanisms of RNP assembly is central to our understanding of the control of viral transcription and replication and the dependence of these processes on the host cell. ...

In order to combine the qualities of inorganic and organic ion exchangers, a stable material with good chromatographic properties, a new inorganic-organic ion exchanger Titanium (IV) phosphosulphosalicylate(TiPSS) was synthesized. Sample-1 was chosen for detailed ion exchange studies due to its highest ion exchange capacity, good appearance and chemical stability. Characterization of the resin ...

Inorganic lead ion in micromolar concentrations inhibits Electrophorus electroplax microsomal (Na+ + K+)-adenosine triphosphatase ((Na+ + K+)-ATPase) and K+-p-nitrophenylphosphatase (NPPase). Under the same conditions, the same concentrations of PbCl2 that inhibit ATPase activity also stimulate the phosphorylation of electroplax microsomes in the absence of added Na+. Enzyme activity is protect...

lead (pb2+) is a well known neurotoxin that was frequently found in the environment and chronic exposure to lead has been matter of public health. in the present study the effect of lead on spatial memory in developmentally exposed rats and their dams in morris water maze task were investigated. female rats were divided into three groups and two groups exposed to 250 and 750 part per million (p...

In an effort to better understand the consequences of lead (Pb2+) on skeletal growth, the effects of Pb2+ were investigated using ROS 17/2.8 bone-like cells in vitro. These studies revealed that Pb2+ (4.5 x 10(-6) M -4.5 x 10(-7) M) has little or no effect on cell shape except when added immediately following seeding of the cells. However, proliferation of ROS cells was inhibited, in the absenc...

a simple, rapid and economic procedure was presented to remove lead(ii) from aqueous solution under the optimized conditions. it is based on the sorption of pb2+ ions from aqueous solutions onto limestone fines (ls), which is an inexpensive and widespread over the globe, followed by flotation with oleic acid (hol) surfactant. the different parameters (namely: solution ph, sorbent, surfactant an...

Divalent metal ions promote hydrolysis of RNA backbones generating 5'OH and 2';3'P as cleavage products. In these reactions, the neighboring 2'OH act as the nucleophile. RNA catalyzed reactions also require divalent metal ions and a number of different metal ions function in RNA mediated cleavage of RNA. In one case, the LZV leadzyme, it was shown that this catalytic RNA requires lead for catal...