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OBJECTIVES To develop and evaluate a rapid method for the diagnosis of malaria infection by microscopy of stained blood films. SUBJECTS AND METHODS Blood specimens were collected from randomly selected confirmed malaria cases (n = 75) and suspected malaria cases (n = 175). The microscopy was done on each set of blood films stained by modified and the standard Giemsa staining methods. RESULT...

In a case of neuroblastoma the presence of an abnormal blood constituent was suspected from the raised erythrocyte sedimentation rate, sludging of the red cells, marked rouleaux formation, an atypical Leishman stain, increased plasma viscosity, and a distorted protein electrophoresis pattern. The abnormal constituent was shown to be a mucopolysaccharide which was either hyaluronic acid or chond...

Recent developments of the aerodynamics models for the comprehensive analysis CAMRAD II are described, particularly the unsteady aerodynamic models and dynamic stall models, and the free wake geometry calculation. Three models for the unsteady aerodynamic loads in attached flow are implemented: from incompressible thin-airfoil theory, from ONERA EDLIN, and from Leishman-Beddoes. Five dynamic st...

Trichomonas vaginalis, a flagellate protozoan parasite commonly found in the human genitourinary tract, is transmitted primarily by sexual intercourse. Diagnosis is usually by in vitro culture method and staining with Giemsa stain. There are laboratories that use Gram stain as well. We compared the use of modified Field's (MF), Giemsa, and Gram stains on 2 axenic and xenic isolates of T. vagina...

fever were met with attending the Dinajpur dispensary, the blood of which was examined for malarial parasites, while on two occasions a number of chronic fevers in the most feverish part of the district were examined by means of spleen puncture for the recently described parasite-like bodies found last year in chronic fevers lay Leishman and Donovan, and more recently in a casg from Sylhet by M...

Helicobacter pylori (Hp) gastritis is a worldwide problem significantly associated with duodenal and gastric peptic ulcer disease, gastric carcinomas, and MALT-type lymphomas. A simple, rapid, reproducible, reliable, and inexpensive stain to detect the organism in gastric biopsy specimens is thus of great value. To assess the reliability and cost-effectiveness of a novel Alcian yellow-toluidine...

Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis cariniipneumonia, it does not normally stain the cyst wall. For detection ofthe cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining wit...

Background: Laboratory diagnosis of malaria is currently recommended for the confirmation of the disease before management. The two most common techniques in use for the diagnosis of malaria parasite in Sudan are Giemsa stained smears and Rapid diagnostic tests(RDTs). This study was carried out to compare microscopy ( Giemsa and Acridine orange stain ) and RDTs as effective tools for the diagno...

Disease caused by protozoan parasites of the genus Leishmania, named in 1901 for British Army doctor William Leishman, who developed a stain to detect the agent. It is transmitted by the bite of certain species of sand fly, including the genus Lutzomyia in the New World and Phlebotomus in the Old World.