PURPOSE To explore expression and function of astrocyte elevated gene-1 (AEG-1) in human retinoblastoma (RB). METHODS The expression of AEG-1 in histological sections of human RBs and in RB cell lines was examined using immunohistochemical staining and RT-PCR and Western blotting respectively. We knocked down AEG-1 gene levels by AEG-1-siRNA lentivirus transfection of human RB cell lines SO-R...

methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...

Introduction: Currently, short interfering RNA (siRNA) is an experimental tool in molecular biology to study gene function cell culture and vivo model of organisms (mouse chicken embryos, etc.) Method: Lentivirus production technique by human embryonic kidney 293T lines (HEK293T) with two (2) sites 8664/P201 2122/Pll.3.7 the sphingosine 1 phosphate receptor endothelial differentiation (S1P1/ Ch...

Lentivirus escape from neutralizing antibodies (NAbs) is not well understood. In this work, we quantified antibody escape of a lentivirus, using antibody escape data from horses infected with equine infectious anemia virus. We calculated antibody blocking rates of wild-type virus, fitness costs of mutant virus, and growth rates of both viruses. These quantitative kinetic estimates of antibody e...