The effects of clofibrate administration were studied in rats made hyperlipidemic by the feeding of diets high in sucrose. Within 12 hr of administration of clofibrate, there was a marked decrease in the concentration of serum high density lipoproteins but no change in the concentrations of the low and very low density lipoproteins. Between 2-4 days of treatment, the concentration of the very l...

Low-density lipoproteins (LDLs) reconstituted with a multifunctional mitochondrion-targeting photosensitizer are able to achieve fluorescence-feedback photodynamic therapy of LDL receptor-overexpressing cancer cells.

Low-density (LD) lipoproteins inhibit phytohaemagglutinin-enhanced turnover of phosphatidylinositol in human peripheral lymphocytes. Turnover was assessed by 32p incorporation into phospholipids and by loss of 32p from [32P]phosphatidylinositol. Inhibition of lipid turnover by LD lipoproteins is not the result of a change in the amount of phytohaemagglutinin required for maximum cellular respon...

OBJECTIVE Experiments in cells and animal models show that lipoprotein lipase (LpL) bound to apolipoprotein (apo)B lipoproteins enhances their uptake by receptor mediated pathways. It is unknown whether this pathway is important in humans. METHODS AND RESULTS ApoB lipoproteins with LpL were isolated from normal subjects after oral fat loading by immunoaffinity chromatography and were further ...

Structural lipids, primarily phospholipids and sterols, as well as energy-storage lipids such as triacylglycerols are insoluble in water. In the aqueous fluids of animals, they are carried as lipoproteins, complexes of lipids bound to polypeptides. These watersoluble lipoproteins can be separated into different classes, depending upon their hydrated densities. These classes include very low den...

The push-pull solvatochromic pyrene derivatives PA and PK have been applied to the study of model membrane vesicles, cells purified human serum lipoproteins, using both confocal fluorescence microscopy spectroscopy. These polarity-sensitive probes provide information similar that obtained by Laurdan or Prodan, i.e. mainly lipid order in biomembranes, but they essential advantage being excitable...

Low density human plasma lipoproteins S(f) 17+, S(f) 13, and S(f) 6, high density lipoproteins 2 and 3, and a lipoprotein-free plasma protein fraction were isolated from human plasma by ultracentrifugal methods. It was found that human plasma lipoproteins are immunochemically distinct from the non-lipoprotein containing plasma protein fraction. Lipoprotein fractions of a given hydrated density,...

Radioiodinated apolipoprotein E, added in small amounts to rat serum, rapidly associates with the serum very low density lipoproteins and high density lipoproteins in proportion to their content of endogenous and apolipoprotein E. The labeled lipoproteins can be separated, without ultracentrifugation, by molecular sieve chromatography. When these labeled lipoproteins are injected intravenously ...

Suckling rat plasma contains (in mg/dl): chylomicrons (85 +/- 12); VLDL (50 +/- 6); LDL (200 +/- 23); HDL1 (125 +/- 20); and HDL2 (220 +/- 10), while lymph contains (in mg/dl): chylomicrons (9650 +/- 850) and VLDL (4570 +/- 435) and smaller amounts of LDL and HDL. The lipid composition of plasma and lymph lipoproteins are similar to those reported for adults, except that LDL and HDL1 have a som...

Plasma triglyceride (TG)-rich lipoproteins are related to high density lipoprotein (HDL)-cholesterol and risk of coronary artery disease (CAD). Two major hypotheses on the role of HDL in the development of CAD have been proposed: a 'causalist' view assigns a protective effect against atherosclerosis to HDL and a 'non-causalist' view states that HDL do not interfere directly with development of ...