The ultrasensitive determination of the carbohydrate components of glycoconjugates is challenging because of their low concentrations in complex biological mixtures and the chemical complexity of glycan chains. A major limitation in the field of glycobiology has been a lack of requisite analytical platforms for biologically active, therapeutic carbohydrates (e.g., glycosaminoglycans (GAGs) and ...

Introduction: Since currently no sensitive and specific biomarkers for early detection of lung adenocarcinoma (AD) exist and the majority of AD patients are diagnosed at late stages of disease, the development of effective screening tests for early-stage lung AD is urgently needed. Serum microRNAs (miRNAs) have been documented as novel noninvasive biomarkers in tumor diagnosis; thus, we studied...

Simulation of gamma and hadron showers in the energy range 100GeV- 25TeV has been carried out. For a big ideal Cherenkov light array, characteristics of lateral distribution of Cherenkov photon density have been studied. Results from this ideal detector are compared with those simulated for a real TUNKA- like array to investigate experimental potential of using existence of a hump in lateral di...

Aims. This paper extends the treatment of system equivalent flux density (SEFD), discussed in our earlier to interferometric phased array telescopes. The objective is develop an SEFD formula involving only most fundamental assumptions that readily applicable interferometer radio observations. Our aim compare resultant expression against often-used root-mean-square (rms) approximation, ${\rm{SEF...

Background. Parkinson's disease (PD) is mostly characterized by alpha-synuclein (SNCA) aggregation and loss of nigrostriatal dopamine-containing neurons. In this study a novel SNCA multiplication is described in two siblings affected by severe parkinsonism featuring early onset dyskinesia, psychiatric symptoms, and cognitive deterioration. Methods. SNCA dosage was performed using High-Density C...

The epidemiology of malaria in "low-transmission" areas has been underestimated. Molecular detection methods have revealed higher prevalences of malaria than conventional microscopy or rapid diagnostic tests, but these typically evaluate finger-prick capillary blood samples (∼5 μl) and therefore cannot detect parasite densities of <200/ml. Their use underestimates true parasite carriage rates. ...

BACKGROUND Hepatitis C virus (HCV) has been reported to regulate cellular microRNAs. The HCV core protein is considered to be a potential oncoprotein in HCV-related hepatocellular carcinoma, but HCV core-modulated cellular microRNAs are unknown. The HCV core protein regulates p21(Waf1/Cip1) expression. However, the mechanism of HCV core-associated p21(Waf1/Cip1) regulation remains to be further...

Excessive subconjunctival scarring is the main reason of failure of glaucoma filtration surgery. We analyzed conjunctival and systemic gene expression patterns after non penetrating deep sclerectomy (NPDS). To find expression patterns related to surgical failure and their correlation with the clinical outcomes. This study consisted of two consecutive stages. The first was a prospective analysis...

Several techniques have been developed to screen mismatch repair (MMR) genes for deleterious mutations. Until now, two different techniques were required to screen for both point mutations and large rearrangements. For the first time, we propose a new approach, called ‘‘quantitative PCR (qPCR) high-resolution melting (HRM) curve analysis (qPCR-HRM),’’ which combines qPCR and HRM to obtain a rap...