BACKGROUND Standardization of clinical measurements of C-reactive protein (CRP) is based on the availability of reference materials. The serum protein reference material ERM-DA470 was used as a calibrant for various commercially available immunoassays but has now been exhausted. The recently released ERM-DA470k/IFCC was intended to fully replace ERM-DA470. However, the new material was not suit...

OBJECTIVE Glutaraldehyde is currently used in bovine pericardium bioprosthesis to improve mechanical and immunogenic properties. Lyophilization is a process that may decrease aldehyde residues in the glutaraldehyde treated pericardium decreasing cytotoxicity and enhancing resistance to calcification. The aim of this study is to evaluate bioprosthetic heart valves calcification in adolescent she...

Dimethylsulfoxide (DMSO)-quenched hydrogen/deuterium (H/D)-exchange is a powerful method to characterize the H/D-exchange behaviors of proteins and protein assemblies, and it is potentially useful for investigating non-protected fast-exchanging amide protons in the unfolded state. However, the method has not been used for studies on fully unfolded proteins in a concentrated denaturant or protei...

Ahn, T. H. (University of California, Los Angeles), H. Nishihara, Charles M. Carpenter, and G. V. Taplin. Viability and metabolism of Staphylococcus aureus after freezing, lyophilization, and gamma irradiation. J. Bacteriol. 88:545-552. 1964.-Reproductive viability and oxidative activity on various substrates (alanine, glutamic acid, glucose, lactic acid) were studied in Staphylococcus aureus c...

Recent developments in tissue engineering approaches frequently revolve around the use of three-dimensional scaffolds to function as the template for cellular activities to repair, rebuild and regenerate damaged or lost tissues. While there are several biomaterials to select as three-dimensional scaffolds, it is generally agreed that a biomaterial to be used in tissue engineering needs to posse...

A procedure for the preparation and distribution of simulated water specimens for coliform density testing is described. Lyophilization of Escherichia coli in a cooked meat-glucose supporting substrate provides stable samples which may be distributed to participants in a proficiency testing program. Logarithmic conversion of the data allows statistical evaluation of the results for inter- and...

The technique described here is a fast and simple method of extracting chloroplast DNA (cpDNA). It overcomes the need for differential centrifugation using density gradients. The leaves do not have to be kept in the dark and lyophilized before extraction, but lyophilization is still possible. The chloroplasts are specifically lysed in a cell extract of leaves, using a non-ionic detergent. After...

Ubiquinone, Lyophilization, N-Methyl Phenazonium Methosulphate, Kinetics, Chromatophores 1. The effects of lyophilization and the extraction of ubiquinone-10 on the kinetics of electron transport in Rhodopseudom onas capsulata Ala pho+ have been investigated. 2. Lyophilization reduced the amount of ferrocytochrome c2 photo-oxidized on a microsecond time scale following a single excitation. 3. L...

Lyophilizatin of 13 cyanobacterial cultures belonging to seven genera was attempted in a variety of suspending substances. All organisms survived lyophilization when suspended in lamb serum. Some of the organisms could be successfully lyophilized in horse serum, beef serum, fetal bovine serum, or human ascites fluid. With the exception of Nostoc muscorium, none of the organisms survived lyophil...