Bryophytes (liverworts, hornworts and mosses) comprise the three earliest diverging lineages of land plants (embryophytes). Marchantia polymorpha, a complex thalloid Marchantiopsida liverwort that has been developed into a model genetic system, occupies a key phylogenetic position. Therefore, M. polymorpha is useful in studies aiming to elucidate the evolution of gene regulation mechanisms in p...

Three new aseptic cultures of Marchantia polymorpha from different collecting sites have been induced from the gametophytes. The formation of bisbibenzyls in differentiated cultures and cell suspension cultures was investigated. We observed that the production of the main bisbibenzyl, Marchantin A was induced by lack of nitrate and the addition of cupric sulfate to the medium. The comparison of...

The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- and Agrobacterium-mediated transformation methods. Here, we report a simplified method for Agrobacterium-mediated transformation of sporelings, and it is termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). ...

Plants have evolved several ways to remain dormant until the time is right for growth. For example, seeds maintain dormancy through the antagonistic effects of the phytohormones abscisic acid (ABA) and gibberellic acid. Auxin (indole-3-acetic acid [IAA]), in turn, regulates ABA-induced seed dormancy through its interaction with the ABA signaling pathway (Liu et al., 2013). Dormancy is also cruc...

The genera Mima and Herellea, members of the tribe Mimeae (De Bord, 1942), contain organisms which morphologically resemble the Neisseriae upon primary isolation. Since they are being isolated in increasing numbers from the spinal fluid of patients with acute meningitis, a method for their differentiation from the Neisseriae would be advantageous. Schaub and Hauber (1948) classified a gramnegat...

Isolates of Mima polymorpha and Herellea vaginicola obtained from retail food products were investigated for their ability to hydrolyze proteins, starch, and lipids as well as for their ability to initiate growth under varying conditions of pH, sodium chloride concentration, and temperature. None of the isolates hydrolyzed starch and a few hydrolyzed proteins, whereas most were actively lipolyt...

The vast majority of land plants develop gas-exchange tissues with intercellular spaces (ICSs) connected directly to the air. Although the developmental processes of ICS have been described in detail at the morphological and ultrastructural level in diverse land plants, little is known about the molecular mechanism responsible for ICS formation. The liverwort Marchantia polymorpha develops a mu...

The antimicrobial activity of methanol extracts three thalloid liverworts, Marchantia polymorpha, Conocephalum conicum and Pellia endiviifolia bis-bibenzyl marchantin A, the most dominant compound in extract M. have been investigated this research. 1H-NMR spectroscopy revealed that polymorpha P. liverwort contain terpenes, oils, sugars bis-bibenzyls, while these specific macrocyclic compounds w...