To further dissect the genetic differences between the checkpoint pathway following S-phase cdc arrest versus DNA damage, a genetic screen was performed for checkpoint mutants that were unable to arrest mitosis following cell-cycle arrest with a temperature-sensitive DNA polymerase delta mutant, cdc20-M10. One such checkpoint mutant, rad17-d14, was found to display the cut phenotype following S...

The sensitizing effects of wild-type p53 on X-ray-induced cell death and on heat-induced apoptosis in M10, a radiosensitive and Trp53 (mouse p53 gene)-mutated lymphoma cell line which dies through necrosis by X-irradiation, were investigated using three M10 derived transfectants with wild-type TP53 (human p53 gene). Cell death was determined by colony formation and/or dye exclusion test, and ap...

Methods Pts with active pJIA were enrolled in one of the following trials: M10-444 (ages 2 to <4 or ≥4 weighing <15 kg in US, EU), M10-240 (ages 4-17 in Japan), DE038 (ages 4-17 in US, EU) or the STRIVE (P10-262) registry (ages 4-17 in US, EU, and Australia). Pts received ADA±methotrexate. Vaccinations were administered based on the judgment of the study investigator or the treating physician. ...

Fusarium oxysporum JCM11502 expresses a denitrifying (nitrate (NO(3)(-))-respiring) mechanism and can thrive under oxygen (O(2)) limitation. The fungus reduces NO(3)(-) to nitrite at the initial step of denitrification. In this study, we cloned the gene coding NADH-NO(3)(-) reductase (NADH-Nar) (niaD) from F. oxysporum JCM11502. The niaD gene complemented the defective NO(3)(-) assimilation by ...

Resistance of the indigenous strains P. jadinii M9 and P. anomala M10, to high Cr(6+) concentrations and their ability to reduce chromium in culture medium was studied. The isolates were able to tolerate chromium concentrations up to 104 μg mL(-1). Growth and reduction of Cr(6+) were dependent on incubation temperature, agitation, Cr(6+) concentration, and pH. Thus, in both studied strains the ...

The primary attachment to polymer surfaces followed by accumulation in multilayered cell clusters leads to production of Staphylococcus epidermidis biofilms, which are thought to contribute to virulence in biomaterial-related infections. We isolated Tn917 transposon mutants of biofilm-producing S. epidermidis 13-1, which were completely biofilm negative. In pulsed-field gel electrophoresis no o...

Absorption, metabolism, and excretion of paliperidone, an atypical antipsychotic, was studied in five healthy male subjects after a single dose of 1 mg of [(14)C]paliperidone oral solution ( approximately 16 microCi/subject). One week after dosing, 88.4 to 93.8% (mean 91.1%) of the administered radioactivity was excreted: 77.1 to 87.1% (mean 79.6%) in urine and 6.8 to 14.4% (mean 11.4%) in the ...