Several yeast strains degrading malic acid as a sole carbon and energy source were isolated from Korean wine pomace after enrichment culture in the presence of malic acid. Among them, the strain designated as KMBL 5774 showed the highest malic acid degrading ability. It was identified as Issatchenkia orientalis based on its morphological and physiological characteristics as well as the nucleoti...

Clostridium thermocellum produces ethanol as one of its major end products from direct fermentation of cellulosic biomass. Therefore, it is viewed as an attractive model for the production of biofuels via consolidated bioprocessing. However, a better understanding of the metabolic pathways, along with their putative regulation, could lead to improved strategies for increasing the production of ...

The activities of the mitochondrial NAD(P)+- and NADP+-dependent malic enzymes were measured in 11 tissues of the male Sprague-Dawley rat. The NAD(P)+-dependent malic enzyme was present in small intestinal mucosa, spleen, thymus, lung, and testis. Each of these tissues contain cells that are undergoing active rates of renewal. The NADP+-dependent malic enzyme was not confined to tissues undergo...

Malic enzyme catalyzes the reversible oxidative decarboxylation of malate to pyruvate and CO(2). The Saccharomyces cerevisiae MAE1 gene encodes a mitochondrial malic enzyme whose proposed physiological roles are related to the oxidative, malate-decarboxylating reaction. Hitherto, the inability of pyruvate carboxylase-negative (Pyc(-)) S. cerevisiae strains to grow on glucose suggested that Mae1...

ment of the rat. 'Malic' enzymes from the livers of normal and hyperthyroid rats were found to be kinetically, electrophoretically and immunologically identical. Hence the increased activity found in hyperthyroid rat liver was due to an increase in the same form of enzyme protein as in normal liver. 'Malic' -enzyme was therefore purified from the livers of hyperthyroid rats by a modification of...

Rat liver malic enzyme (EC 1.1.1.40) was purified from livers of rats fasted and refed a high sucrose diet containing 1% desiccated thyroid powder. The purification was accomplished by a six-step procedure. The specific activity of the purified enzyme was increased 181-fold above that of the initial high speed supernatant of liver extracts. Slight additional purification of malic enzyme was ach...

Best, Audrey N. (University of Georgia, Athens), and W. J. Payne. Preliminary enzymatic events in asparagine-dependent denitrification by Pseudomonas perfectomarinus. J. Bacteriol. 89:1051-1054. 1965.-The initial events leading to denitrification by Pseudomonas perfectomarinus grown anaerobically in the presence of NO(3) (-) were investigated. Conversion of asparagine to malic acid was demonstr...

Malic enzyme was isolated and purified from mature apple fruits (Malus sylvestris, Miller) by utilizing procedures probably applicable to other soluble enzymes in this and similar tissues.The physical properties of apple fruit malic enzyme are similar to those reported for malic enzyme from other plant and animal sources. It is specific for l-malate, TPN and requires a divalent cation for activ...

A sensitive and specific chemical method for the estimation of malic acid in biological materials has long been desired. The condensation of malic acid with orcinol in the presence of concentrated sulfuric acid to form the highly fluorescent homoumbelliferone (7-hydroxyd-methylcoumarin) was reported years ago (1). Although this reaction is relatively specific for malic acid, the quantitative pr...

The occurrence of malic acid in fermented products derived from various fruit juices is common knowledge and it has been generally supposed that this substance was exclusively derived from malic acid preformed in the fruit. The object of the following communication is to establish the fact that I-malic acid is a true product of fermentation by yeast and that significant amounts of the acid are ...