8, 16 or 24 Determinations Version 07/2015 Please pay attention to the differences in comparison to the previous version 10/2013 Complete revision: details on specimen added (page 5), information on performance data and interfering substances added (page 8). Modification and improvement of test procedure (page 6) Page 6: Procedure: Dilution of serum samples 1:200 in one step Sample incubation: ...

Immunoassays are among the most powerful and useful techniques for analysis of biological materials. There are numerous variations in which immunoassays can be performed. Coupled with enzyme, using chromogenic substrates, the enzyme immunoassay technique is used to trace the target antigen in tissues. The technique also is used to measure the concentration of antigen in tissue extracts. This ch...

partially fed (4 -5 days) h .a. anatolicum (iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (gs), gut membrane antigen (gm), triton extracted gut membrane antigen ( tx-gm ) and sodium dodecyl sulphate treated triton extracted gut membrane antigen (sds-tx-gm). the new zealand white rabbits were immunized with gs, gm, tx- gm...

in leishmania (l.) major-infected balb/c mice th2-type cells results in disease progression, whereas c57bl/6-infected mice mount a th1-type response, which leads to control of the infection. th2 response correlates with igg1 whereas, th1 response supports switching to igg2a subclasses. since igg isotype-dominated response depends on different cd4 + t cell subsets, we studied the antigenic profi...

umbelliprenin is a prenylated compound, which belongs to the class of sesquiterpene coumarins. it is extracted from dried roots of ferula szwitsiana collected from the mountains of golestan forest (golestan province, north of iran). induction of apoptosis in jurkat t-cll cells has been previously shown. in this study, effect of umbelliprenin on proapoptotic caspases (caspase-8 and -9) and antia...

objective(s):antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. the aim of this study was to produce polyclonal antibody against a synthetic peptide derived from n-terminal region of β-actin protein to be used as a protein loading control in western ...

background and aims: viroids are smallest known plant pathogens and cause several economically significant diseases. until recently, viroid detection relied mainly on biological tests and indexing. today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (rt-pcr) are being used for detection and diagno...