Background: Despite great advances achieved in the field of in vitro oocyte maturation (IVM), almost all IVM media are supplemented with undefined component which not only pose the risk of pathogen transmission but also hinders our knowledge of molecular mechanisms of maturation. One of these components is serum that containing various concentration of unknown molecules affecting cellular proce...

This study was designed to clarify bilaterally effect of long-time varicocele on left and right testes and to identify the effect of varicocele on maturation divisions during spermatogenesis. Moreover, the percentage of seminiferous tubules (STs) with I-VIII and IX-XIV stages of spermatogenesis and sperm parameters such as sperm motility and abnormality types were evaluated. The left varicocele...

background: when we perform surgery in utero, lungs have an appropriate time to decrease magnitude of hypoplasia, with surgery in uterus. methods: six time-dated single-fetus ewes were selected to induce diaphragmatic hernia. fetal lambs were divided proportionally into 2 groups, namely group 1, diaphragmatic hernia and tracheal ligation (tl group), and group 2, diaphragmatic hernia only (nl gr...

Background: Oocyte maturation is usually defined as the period of progression from the first to the second meiotic arrest and involves coordinated nuclear and cytoplasmic modification. Ca2+ signals are known as important regulators of oocyte maturation. Extracellular Ca2+ is important for first polar body formation, normal meiosis kinetics, and for preserving the chromosome and spindle configur...

Background We have previously reported the effect of Anthocyanin and cAMP modulators on maturation and apoptosis of sheep oocytes. In this study, we investigated the reduction capacity and apoptotic gene expression results at MII sheep oocytes. MaterialsAndMethods Good ovine Cumulus-oocyte complexes (COCs) were transferred to Pre-IVM medium, then to two type IVM mediums: 1) 0.1μg/ml Delphinidin...

background: oocyte maturation and subsequent in vitro production (ivp) of embryos are affected by diverse groups of chemicals in maturation medium which are needed for successful mammalian oocyte maturation during which the dramatic cytoplasmic and nuclear reprogramming events take place. this study was designed to evaluate the effects of protein source (fetal bovine serum, fbs, and bovine seru...