Disulfide bonds play a crucial role in proteins, modulating their stability and constraining their conformational dynamics. A particularly important case is that of proteins that need to withstand forces arising from their normal biological function and that are often disulfide bonded. However, the influence of disulfides on the overall mechanical stability of proteins is poorly understood. Her...

Structural integrity as well as mechanical stability of the parts of a molecular motor are crucial for its function. In this study, we used high-resolution force spectroscopy by atomic force microscopy to investigate the force-dependent opening kinetics of the neck coiled coil of Kinesin-1 from Drosophila melanogaster. We find that even though the overall thermodynamic stability of the neck is ...

Introduction:  Stereotactic  radiosurgery  is  a  technique  for  the  treatment  of  intracranial  lesions  with  highly  collimated  x-ray  beam.  Linac-based  radiosurgery  is  currently  performed  by  two  techniques:  couch or pedestal mounted. In the first technique, a device is required to affix patient's head to a couch  and  moreover  to  translate  it  accurately.  Structure  of  suc...

Background: Mesenchymal stem cells (MSCs) are multipotent cells, capable of differentiating into different cell lines.They can sense their surrounding biochemical and biophysical factors, which play major roles in their differentiation toward different phenotypes. Therefore, the exposure of these cells to endothelial growth factor (VEGF) as well as hemodynamic biomechanical forces, which act on...

Mechanical unfolding and refolding may regulate the molecular elasticity of modular proteins with mechanical functions. The development of the atomic force microscopy (AFM) has recently enabled the dynamic measurement of these processes at the single-molecule level. Protein engineering techniques allow the construction of homomeric polyproteins for the precise analysis of the mechanical unfoldi...

Create three-dimensional network of siloxane polymer in the epoxy resin is an appropriate mechanical properties .Dispersion of nanoclay in these coating was prepared by ultrasonication method. Mechanical properties of nanocomposit coatings such as abrasion, hardness and adhesion was increased by addition of nanoclay montmorillonite. Different percentages of nanoclay in coating showed that even ...