During the Folin Ciocalteu (F-C) micro-assay for the determination of phenolics in the presence of methanol, fine solids can form. In a previous paper, we hypothesized that the interference from alcohol on the F-C reaction can be minimized depending on the particular procedure used to reach the alkalinity condition. In order to demonstrate our hypothesis we studied, by spectrophotometrically mo...

BACKGROUND Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed with commercial base-catalytic process. Enzymatic process is preferable to convert waste fat into biodiesel since enzyme can catalyze both esterification of free fatty acid and...

Liquid methanol shows one- and two-dimensional (1D/2D) hydrogen bond (HB) networks, and liquid water shows three-dimensional (3D) HB networks. We have clearly found three different local structures around the methyl group of methanol-water binary solutions (CH3OH)X(H2O)1-X at different concentrations in C K-edge soft X-ray absorption spectroscopy (XAS). With the help of molecular dynamics simul...

An eight-month-old infant ingested at least 3.5 g of methanol when he accidentally received 5 mL of amoxicillin suspended in 70% methanol. The serum methanol concentration 8 h later was 9.7 mmol/L (310 mg/L) and the formate concentration 23 mmol/L (1.0 g/L). At 18 h after the ingestion, total CO2 had decreased to 6.8 mmol/L. Throughout the second day, 21-32 h after the ingestion, the methanol c...

Canarium odontophyllum (CO) Miq. has been considered as one of the most sought-after plant species in Sarawak, Malaysia, due to its nutritional and pharmacological benefits. This study aimed to evaluate the pharmacodynamic interaction of crude methanol and acetone extracts from CO leaves in combination with oxacillin, vancomycin, and linezolid, respectively, against MRSA ATCC 33591 as prelimina...

Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoters which are regulated by methanol. However, the use of methanol has several disadvantages, which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) are focusing on methanol mixed feeding strategies. This work aimed to develop a new experimental method and compare the effect of v...

BACKGROUND The incidence of methanol (CH3OH) intoxication differs enormously from country to country. Methanol intoxication is extremely rare in the Dutch population. Even a low dose can already be potentially lethal. Patients are conventionally treated with hemodialysis. Therefore we'd like to present a report of a foreign sailor in Rotterdam who accidentally caused himself severe methanol int...

OBJECTIVE To evaluate in vitro antibacterial effectiveness of five medicinal plants used by an Indian aborigine, against 8 multidrug-resistant (MDR) enteropathogenic bacteria isolated from clinical samples of under-5 hospitalized children. METHODS Antibiotic sensitivity patterns of eight clinically isolated strains of enteropathogenic bacteria, Enterobacter aerogenes, Escherichia coli, Klebsi...