INTRODUCTION Pneumocystis jirovecii dihydropteroate synthase (DHPS) gene mutations' (55th and 57th codon) association with prior sulfa prophylaxis failure has been reported from both developed and developing countries. We conducted a prospective study to determine the prevalence of P. jirovecii DHPS mutations from 2006 to 2009 on P. jirovecii isolates obtained from HIV-infected patients with a ...

Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for kappa and lambda light immunoglobulin chains and C...

Abbreviations: AIDS, acquired immune deficiency syndrome; ARDS, adult respiratory distress syndrome; BAL, bronchoalveolar lavage; CSF, cerebrospinal fluid; CMV, cytomegalovirus; CNS, central nervous system; CT, computed tomography; DNA, deoxyribonucleic acid; EIA, enzyme immunoassay; GMS, Grocott methenamine-silver; HIV, human immunodeficiency virus; HPLC, high performance liquid chromatography...

In this paper, we show that first-principle calculations using a van der Waals density functional (vdW-DF) [M. Dion, H. Rydberg, E. Schroder, D. C. Langreth, and B. I. Lundqvist, Phys. Rev. Lett. 92, 246401 (2004)] permit the determination of molecular crystal structure within density functional theory (DFT). We study the crystal structures of hexamine and the platonic hydrocarbons (cubane and ...

The protonation constant of hexamethylenetetramine (urotropine) was determined by a potentiometric and a spectrophotometric method. The calculations gave log K(HL) (concentration constants): 4.89 at mu = 0.1 and 5.05 at mu = 0.5. The temperature was 25 degrees and potassium chloride was used to adjust the ionic strength.

age related cytochemical changes of thymic endocrine cells were studied in 78 day old chicks at five day interval to age of day 60 employing a panel of cytochemical stains. methenamine silver revealed cell morphology including cell processes distinctly while diamine silver revealed a stronger argentaffinity in these cells. the cells had greater affinity for diamine silver compared to methenamin...

Age related cytochemical changes of thymic endocrine cells were studied in 78 day old chicks at five day interval to age of day 60 employing a panel of cytochemical stains. Methenamine silver revealed cell morphology including cell processes distinctly while diamine silver revealed a stronger argentaffinity in these cells. The cells had greater affinity for diamine silver compared to methenamin...

OBJECTIVES The aim of this study was to investigate antimicrobial prescribing in nursing homes in countries across Europe. METHODS Point prevalence studies were completed in April and November 2009 in 85 nursing homes in 15 European countries and two UK administrations. RESULTS A total of 10,388 and 9430 residents participated in April and November 2009, respectively. The mean prevalence of...

BACKGROUND Residents in nursing homes have a higher risk of developing infections that require antibiotic treatment than elderly people living at home. Use of antibiotics may cause adverse effects and result in the development of antimicrobial resistance. MATERIAL AND METHOD Data on healthcare-associated infections and antibiotic use in 540 Norwegian nursing homes were retrieved from the Norw...