aquatic animals are important resources for extraction of valuable compounds such as enzymes. in this study, lipase was extracted from the intestine of rainbow trout. samples were defatted with acetone several times; ammonium sulphate (60%) was used for precipitation followed by ultrafiltration for concentrating the samples. the final purification of the enzyme was performed by passing of the s...

The possible role of the regulatory light chains (LC2) in in vitro assembly of rabbit skeletal and dog cardiac myosins was examined by formation of minifilaments and synthetic thick filaments. After LC2 was removed, the resulting myosin preparations exhibited little aggregation in 0.5 M KCl and 0.05 M potassium phosphate (pH 6.5). Minifilaments migrated as a single, hypersharp peak during sedim...

MgCl 2 –KCl–NaCl is a promising thermal energy storage (TES) material and heat transfer fluid (HTF) with high operating temperatures of >700°C for next-generation concentrating solar power (CSP) plants. One major challenge future implementation the molten chloride TES/HTF technology arises from presence some corrosive impurities, especially MgOHCl, hydrolysis product hydrated . Even extr...

Polymerase chain reaction (PCR) is a rapid and simple technique with high sensitivity and specificity. Inthe recent years, PCR has been used for rapid detection of viral nucleic acids, such as Humancytomegalovirus (HCMV), whereas, PCR optimization is an important task to be done, especially beforeit’s diagnostic application. Annealing temperature, ion concentration (especially...

This study focuses on the impact of landfill high concentration solutions erosion on geosynthetic clay liner (GCL) materials permeability. The permeation tests on the GCL, submerged using different kinds of solutions with different concentrations, were carried out systematically by taking these chemical solutions as permeant liquids. Based on seasonal variations of ion concentrations in Chenjia...

At low ionic strength, Acanthamoeba myosin-II polymerizes into bipolar minifilaments, consisting of eight molecules, that scatter about three times as much light as monomers. With this light scattering assay, we show that the critical concentration for assembly in 50-mM KCl is less than 5 nM. Phosphorylation of the myosin heavy chain over the range of 0.7 to 3.7 P per molecule has no effect on ...

High translocation speed of a DNA strand through a nanopore is a major bottleneck for nanopore detection of DNA molecules. Here, we choose MgCl2 electrolyte as salt solution to control DNA mobility. Experimental results demonstrate that the duration time for straight state translocation events in 1 M MgCl2 solution is about 1.3 ms which is about three times longer than that for the same DNA in ...

A 190-kDa centrosomal protein interacts with microtubules when Drosophila embryo extracts are passed over microtubule-affinity columns. We have obtained a partial cDNA clone that encodes this protein. Using a fusion protein produced from the clone, we have developed a novel immunoaffinity chromatography procedure that allows both the 190-kDa protein and a complex of proteins that associates wit...

A Gram-positive short rod isolated from a saline soil in China was characterized using a polyphasic approach. This actinobacterium grew over a wide salinity range [0-15 % NaCl, 0-20 % KCl and 0-30 % (MgCl2).6H2O (w/v); optimum concentrations for growth were 5 % NaCl, 5-10 % KCl, 10 % (MgCl2).6H2O]. The optimum growth temperature and pH were 28-30 degrees C and 7.0-8.0, respectively. Chemotaxono...

The ATP-dependent phosphoenzyme formation and its reversal were studied at 0 degrees C and pH 7.0 in the ATPase of sarcoplasmic reticulum. Addition of KCl or several other salts (approximately 100 mM) decreased the maximum rate of ADP-induced dephosphorylation of phosphoenzyme as well as the apparent affinity of the phosphoenzyme toward ADP. High ATP had a similar effect on the latter, whereas ...