Field experiments are described involving nitrogen fertilizer in wheat field to research its effect on microbial quantity and enzyme activity. The results showed that 250 kg N hm applied in wheat field (1) can increase bacteria, fungi, actinomycetes quantity by 39.9%, 56.7% and 70.5% compare to 0 kg N hm. (2) Similarly, the activities of soil urease, catalase and FDA expressed 60.8%, 18.3% and ...

The activities of extracellular enzymes, the proximate agents of decomposition in soils, are known to depend strongly on temperature, but less is known about how they respond to changes in precipitation patterns, and the interaction of these two components of climate change. Both enzyme production and turnover can be affected by changes in temperature and soil moisture, thus it is difficult to ...

It is critical to understand the consequences of environmental change for microbial regulation carbon and nutrient cycling. Specifically, understanding community traits, such as extracellular enzyme activity, can help inform cycling models address knowledge gaps. We analyzed data on activities litter decomposition from an 18-month experiment in which communities were reciprocally transplanted a...

The acylneuraminate pyruvate-lyase gene from Clostridium perfringens was sequenced and found to be most similar to the lyase gene from Haemophilus influenzae. Both the recombinant clostridial enzyme and the native enzyme from pig kidney were purified in larger amounts and characterized. The properties of the porcine lyase are similar to the microbial ones. However, the much higher degree of sim...

Amylase is an important and indispensable enzyme that plays a pivotal role in the field of biotechnology. It is produced mainly from microbial sources and is used in many industries. Industrial sectors with top-down and bottom-up approaches are currently focusing on improving microbial amylase production levels by implementing bioengineering technologies. The further support of energy consumpti...

Fluctuations in climate and edaphic factors influence field decomposition rates and preclude a complete understanding of how microbial communities respond to plant litter quality. In contrast, laboratory microcosms isolate the intrinsic effects of litter chemistry and microbial community from extrinsic effects of environmental variation. Used together, these paired approaches provide mechanisti...