Analysis of the population genetic structure and reproductive strategies of various primate species has been facilitated by cross-species amplification (i.e., the use of microsatellite markers developed in one species for analysis of another). In this study we screened 47 human-derived markers to assess their utility in the white-handed gibbon (Hylobates lar). Only eight produced accurate, reli...

Genetic diversity is a key component of ecosystems. The aim of the present study was to evaluate the genetic diversity of Iranian native honeybee colonies (Apis mellifera meda L.) located in the northern region. Colonies from 24 locations have been analyzed using microsatellite markers. Samples were collected from Caspian Sea southern area (north of Iran). Six microsatellite markers (A28, A29, ...

Microsatellites, also known as simple sequence repeats (SSRs), are among the most commonly used marker types in evolutionary and ecological studies. Next Generation Sequencing techniques such as 454 pyrosequencing allow the rapid development of microsatellite markers in nonmodel organisms. 454 pyrosequencing is a straightforward approach to develop a high number of microsatellite markers. There...

The investigation of microsatellite markers has recently superseded that of isoenzymes for many population-biology applications. Microsatellites have the advantages of being dominant, neutral, highly polymorphic and easily scored by high-throughput methods. However, it is necessary to develop a new panel of markers for each group of organisms of interest. Previously, only about 5% of the marker...

the aim of this study was to assess the genetic diversity of iranian turkmen horse using microsatellite markers (hms02, hms03, hms07 and aht04). blood samples were collected from 51 turkmen horses in khorasan shomali province, iran. genomic dna was extracted and then standard polymerase chain reaction performed to amplify the microsatellite markers using specific primers. the pcr products were ...

The applicability of bovine autosomal microsatellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per mar...

UNLABELLED PREMISE OF THE STUDY This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. • METHODS AND RESULTS Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 ma...

Antheraea assama, an economically important and scientifically unexplored Indian wild silkmoth, is unique among saturniid moths. For this species, a total of 87 microsatellite markers was derived from 35 000 expressed sequence tags and a microsatellite-enriched sub-genomic library. Forty individuals collected from Tura and West Garo Hills region of Northeast India were screened for each of thes...

BACKGROUND Retinoblastoma is the most common intraocular tumor in childhood and mutation in the RB1 gene will trigger the tumorigenesis. So far, a wide range of the mutations along the length of RB1 gene have been reported. However, some could not be detected by common detection methods. In such condition, linkage analysis using microsatellite markers is suggested to trace unknown RB1 mutations...

background and objectives: hereditary nonpolyposis colorectal cancer (hnpcc) is an autosomal dominant cancer predisposition syndrome caused by germ-line mutations in dna mismatch repair genes. tumors arising as a result of these mutations display instability in a sequence area known as microsatellites. studies have shown that some bethesda markers (bat25, bat26) are more efficient than others i...