haploid plant per floret (Devaux, 1988). Androgenesis induction in microspores may be affected by various An efficient doubled-haploid production technology that induces factors which cause low induction efficiency and by gehomozygosity can greatly reduce the time and cost of cultivar developnotype dependence (Dunwell, 1985). Most advances toment. Low efficiency of doubled-haploid production pr...

Abstract Background Microspore embryogenesis is potentially the most effective method of obtaining doubled haploids (DH) which are utilized in breeding programs to accelerate production new cultivars. However, regeneration albino plants significantly limits exploitation androgenesis for DH cereals. Despite many efforts, precise mechanisms leading development regenerants have not yet been elucid...

Metabolic engineering was used to disrupt glutamine metabolism in microspores in order to block pollen development. We used a dominant-negative mutant (DNM) approach of cytosolic glutamine synthetase (GS1) gene under the microspore-specific promoter NTM19 to block glutamine synthesis in developing pollen grains. We observed partial male sterility in primary transgenic plants by using light micr...

We analysed the presence of nuclear bodies and particularly Cajal bodies during representative stages of gametophytic and haploid embryogenic development in isolated microspore and anther cultures of a model system (Brassica napus cv. Topas) and a recalcitrant species (Capsicum annuum L. var. Yolo Wonder B). The nuclear bodies domain is involved on several important roles on nuclear metabolism,...

Haploid technologies are used to create homozygous lines for accelerated breeding. We aimed optimize the technology using isolated microspore culture in vitro obtain doubled haploids of carrot (Daucus carota L.).
 studied two varieties with different responsiveness embryogenesis, Altajskaya lakomka and Breeding line 17. Carrot microspores were from buds cultivated liquid nutrient media sup...