A facile and efficient molecularly imprinted polymer (MIP) recognition element of electrochemical sensor was fabricated by directly electro-polymerizing monomer o-phenylenediamine (oPD) in the presence of template quinoxaline-2-carboxylic acid (QCA), based on one-step controllable electrochemical modification of poly(pyrrole)-graphene oxide-binuclear phthalocyanine cobalt (II) sulphonate (PPY-G...

The molecular weight of a DPN-linked isocitrate dehydrogenase preparation, exhibiting a single major band on polyacrylamide disc gel electrophoresis, has been examined by molecular sieve chromatography. Comparison of the elution volume (V,) of the enzyme with reference proteins of known molecular weight on columns of Sephadex G-ZOO and Sepharose 6B permitted calculation of a molecular weight in...

Magnetic molecular imprinted polymers nanozyme MIPs@Fe3O4-NH2 with high specificity and excellent peroxidase-like activity was prepared for colorimetric detection of protocatechuic acid (PCA). Firstly, obtained by self-polymerization dopamine as a functional monomer on the surface Fe3O4-NH2, enabling formation specific recognition cavities. Later, elution PCA using polar solvents resulted in cr...

The molecular weight of a DPN-linked isocitrate dehydrogenase preparation, exhibiting a single major band on polyacrylamide disc gel electrophoresis, has been examined by molecular sieve chromatography. Comparison of the elution volume (V,) of the enzyme with reference proteins of known molecular weight on columns of Sephadex G-ZOO and Sepharose 6B permitted calculation of a molecular weight in...

abstract objective: the aim of this study was to evaluate the efficacy of antibiotic-loaded bone cement in controlling local infection and in regard to its physical characteristics, elastic modulus, and tensile strength in-vitro. methods: acrylic bone cement, based on polymethylmethacrylate (pmma) was mixed with the powder form of three antibiotics, i.e., gentamicin, tobramycin, and cefuroxime ...

In the present study, silver nanoparticles were synthesized by decomposition of AgNO3 via gelcasting. Methacrylamide was used as a low toxic monomer for gel formation. Effect of monomer content (MAM), cross-linker to monomer ratio (MBAM/MAM), silver salt to monomer ratio (AgNO3/MAM), duration of calcination, and temperature of calcination were investigated to optimize the size of nanoparticles....

Enzymes adsorbed to ion exchangers are in many cases specifically desorbed by interaction with their ligands. This phenomenon has been variously called ‘specific elution with substrate’ or ‘affinity elution’ in the literature (von der Haar, 1974). Because the most effective ligands for elution are those containing charged groups identical with those on the ion exchanger (positive groups in the ...

Sample preparation methods tend to be fast, simple, with the use of new adsorbents and lower amounts organic solvents make them more environmentally friendly. In this work, a magnetic solid-phase extraction (MSPE) protocol for quantification six opium alkaloids (OAs) in poppy seeds infusions has been optimised followed by analysis liquid chromatography coupled tandem mass spectrometry (HPLC-MS/...

Objective: The new Ultra-TechneKowt Dry Ship Top Elute 99mTc generator (UTKt-DTE generator; Mallinckrodt Medical, Inc., St. Louis, MO) was devised to facilitate fractionated elution with an ergonomically designed elution shield. Fractionation is accomplished traditionally by visually observing the eluted volume through 2 layers of leaded glass windows located in a lighted elution shield and gen...