BACKGROUND In mouse endometrium, glycogen synthase kinase-3beta (GSK3beta) is a key enzyme controlling nuclear localization of cyclin D1. We developed a functional model of xenografted human endometrium to test whether similar mechanisms are operative in the human by using Lithium chloride (LiCl), an inhibitor of GSK3beta. METHODS Human endometrial samples were obtained from normal volunteers...

It is well known that maternal folate deficiency results in adverse pregnancy outcomes. In addition to aspects in embryonic development, maternal uterine receptivity and the decidualization of stromal cells is also very important for a successful pregnancy. In this study, we focused on endometrium decidualization and investigated whether apoptosis, which is essential for decidualization, was im...

We investigated the effects of a modified Shoutaiwai recipe on integrin β3 and leukemia-inhibitory factor (LIF) in the endometrium of controlled ovarian hyperstimulation (COH) mice during the implantation window. Seventy non-pregnant mice were randomly divided into 3 groups: a traditional medicine (TCM) treatment group (N = 30), an aspirin treatment (N = 30) group, and a control group (N = 10)....

objective: considering the importance of integrin molecules in the implantation and lack of sufficient information in the expression pattern of these molecules in various phases of estrous cycle. it seemed to be necessary to investigate these molecules in mouse endometrial during the various phases of oestrous cycle. materials and methods: female nmri mice (n=15) aged 6-8 weeks were studied. v...

Innate lymphoid cells (ILCs), including NK cells, contribute to barrier immunity and tissue homeostasis. In addition to the role of uterine NK cells in placentation and fetal growth, other uterine ILCs (uILCs) are likely to play roles in uterine physiology and pathology. In this article, we report on the composition of uILCs in the endometrium during the luteal phase and in the decidua during e...

Endometrial carcinoma is the most common gynecologic cancer, yet the mechanisms underlying this disease process are poorly understood. We hypothesized that Lef1 is required for endometrial gland formation within the uterus and is overexpressed in endometrial cancer. Using Lef1 knockout (KO) mice, we compared uterine gland development to wild-type (WT) controls, with respect to both morphology a...

ObjectiveTo identify targets and discover drugs for ovarian endometriosis (OE)DesignA basic study based on a data-driven hypothesis experimental validationSettingCenter Reproductive MedicinePatient(s)/Animal(s)Fourteen patients with OE 7 healthy donors were recruited, 15 female C57/BL6 mice involved.Intervention(s)Samples of lesions normal endometrium obtained. The ITPR1-knockdowned ectopic hum...

background: the aim of this study was to determine the correlation between ultrastructural studies for pinopodes expression after ovarian hyperstimulation and progesterone injection in mice. materials and methods: adult nmri mice were superovulated using human menopasual gonadotropic (hmg) and human chorionic gonadotropic (hcg) hormones; after that, daily injection of progesterone (1 mg/mouse) ...