arnebia pulchra (willd. ex roem. & schult.) edm. is an endangered, perennial member of boraginaceae family. spectrophotometric and lc-mass analysis confirmed the existence of shikalkin derivatives in its root skin and polyphenolic compounds in the inner part of its root. studies on the regeneration of a. pulchra from its callus resulted in successful callus induction in the root explants excise...

an experiment was conducted to study the micropropagation of satureja khuzistanica. the seeds were planted in 1/2ms medium. after germination and growth of plantlets, they were subcultured in fresh medium. then, they cultured into medium supplemented by different concentrations of ba and kinetin. results showed that, the highest proliferation (2.4 shoots) was observed at concentrations of 0.5 m...

Arnebia pulchra (Willd. ex Roem. & Schult.) Edm. is an endangered, perennial member of Boraginaceae family. Spectrophotometric and LC-Mass analysis confirmed the existence of shikalkin derivatives in its root skin and polyphenolic compounds in the inner part of its root. Studies on the regeneration of A. pulchra from its callus resulted in successful callus induction in the root explants excise...

The two commercially important apple rootstocks i.e., MM106 and B9 were micropropagated using a liquid culture system. Three different strengths of 0.8% agar solidified PGR free basal MS medium were first tested to optimize the culture media for both the rootstocks. Full strength medium (MS0) supported maximum in vitro growth, multiplication, rooting and survival under field conditions as oppos...

In vitro shoot multiplication of Merton I. 793, a clonal apple rootstock, was achieved through terminal/axillary bud culture using Murashige and Skoog (MS) basal medium supplemented with 0.5-1.0 mg/L 6-benzyl amino purine (BA) and 0.1 mg/L indole-3-butyric acid (IBA). Time of collection of explants, duration of surface sterilents and growth regulators added in the medium influenced the survival...

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and M...

Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and αnaphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm TDZ and 0.1 mg dm NAA. It was noted t...

A highly efficient protocol for in vitro propagation of Rauwolfia serpentina L. was developed that consisted of callus induction, multiple shoot regeneration and rooting stages. Effect of different plant growth regulators, exclusively or in combination on callus induction, multiple shoot formation and root induction, were observed. Maximum callusing (93.65%) were obtained in leaf and stem expla...

Multiple shoots were obtained from nodal segments of mature trees of Elaeagnus angustifolia L. cultured on MS medium (Murashige and Skoog 1962) supplemented with 0, 0.88 or 2.22 micro M N(6)-benzyladenine. When nodal segments taken from the in vitro proliferated shoots were cultured under the same conditions, additional multiple shoots were obtained. Rooting of the in vitro propagated shoots wa...