OBJECTIVES The purpose of this study was to evaluate the capacity of BioPure MTAD to induce genetic damage in vitro. Genotoxicity was assessed by the single cell gel (comet) assay. METHODS Chinese hamster ovary (CHO) cells or murine fibroblasts cells were exposed to increasing final concentrations ranging from 0.1 a 10%. All treatments were performed for 1 hour at 37 degrees C. The negative c...

OBJECTIVE To evaluate the antimicrobial effect by measuring the minimum inhibitory concentration MIC and minimum bactericidal concentration MBC of propolis, BioPure MTAD, 5% sodium hypochlorite (NaOCl), and 2% chlorhexidine CHX on Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) in vitro. METHODS This study was performed in the Faculty of Dentistry and Pharmacy at Erciye...

Mechanical instrumentation of root canals produces a smear layer that adversely affects the root canal seal. The aim of this study was to evaluate efficacy of MTAD and citric acid solutions used with self-adjusting file (SAF) system on smear layer. Twenty-three single-rooted human teeth were used for the study. Canals were instrumented manually up to a number 20 K file size. SAF was used to pre...

AIM The present study sought to compare the antimicrobial efficacy of 3% NaOCl, Biopure MTAD (Tulsa Dentsply, Tulsa, OK) and Brazilian ethanolic extract of propolis (EEP) against Enterococcus faecalis (E. faecalis). METHODOLOGY The study utilized 55 extracted human permanent teeth with single root canal. The samples were decoronated, instrumented and sterilized. The teeth were infected with E...

Objectives. To evaluate and compare the antifungal efficacy of 1.3% NaOCl/MTAD with 2.5% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX), and iodine potassium iodide (IKI). Materials and Methods. Fifty-two single rooted teeth were used which were divided into four groups with 10 teeth in each group: 2.5% NaOCl, 2% CHX, IKI, 1.3% NaOCl/MTAD, and physiologic saline. Two teeth served...

Aim: To compare the effect of QMix, a mixture of tetracycline, citric acid, and detergent (MTAD), and ethylenediaminetetracetic acid (EDTA) on the microhardness of the root canal dentin. Materials and methods: A total of 30 single-rooted teeth were decoronated at 15 mm from the root apex. Root canal instrumentation using step back technique was done and the specimens were split into two halves ...

The purpose of the study was to evaluate the effect of MTAD in comparison with EDTA as a final rinse on the shear bond strength of Kerr, Apexit and AH plus. Dentine surfaces of 135 extracted human non-carious maxillary premolars were divided into three groups (n = 45). Groups I and II were conditioned with 1.3% NaOCl for 20 min, followed by a final rinse of 17% EDTA for 1 min and MTAD for 5 min...

The aim of this study was to assess the effect of three root canal irrigation solutions on the apical sealing ability of three root canal obturation materials: gutta-percha/AH plus or MM-seal and Resilon/Epiphany SE. A total of 100 teeth with single straight root canals were randomly divided into three equal groups of 30 samples each, with the other 10 teeth (5 positive and 5 negative) used as ...

introduction: the purpose of this in vitro study was to assess the antifungal activity of final canal rinse with either three concentrations of sodium hypochlorite (naocl) (0.5, 2.6 and 6%), two concentrations of chlorhexidine (chx) (2% and 0.2%), mtad, tetraclean, hypoclean and chlor-xtra on candida albicans (c. albicans ) in a human tooth model. methods and materials: two hundred and thirty f...

introduction: root canal disinfection is an important step in regenerative endodontic treatments. an ideal irrigating solution must have high antimicrobial activity and minimum cytotoxicity. this study sought to assess the effect of some irrigating solutions on stem cells from the human apical papilla (scap) after different periods of exposure. methods and materials: stem cells were isolated fr...