Mycoplasma sp. (strain F38) is the causative agent of contagious caprine pleuropneumonia, which is a goat disease of great global concern. Strain F38 belongs to the so-called "Mycoplasma mycoides cluster," and the members of this cluster have many biochemical and serological properties in common, which makes it difficult to differentiate between them by conventional methods. Their phylogenetic ...

The surface carbohydrate structures on the cell membranes of various mycoplasma species have been investigated by using lectins, which are sugar-specific proteins. Carbohydrate structures presumably bound to glycolipids, with both galactose and glucose units, were found to be exposed on the surface of Mycoplasma pneumoniae and its temperature-sensitive mutants, M. mycoides var. mycoides and cap...

Contagious bovine pleuropneumonia (CBPP) is an important disease of cattle in Africa caused by Mycoplasma mycoides subsp. mycoides biotype small colony (MmmSC) and characterized by extensive lesions of pleurisy and pneumonia (13). Among infectious diseases that threaten African cattle, its importance is only second to rinderpest (RP) and, as more and more countries declare being free of RP, CBP...

The clinical course of infection following endobronchial inoculation of three strains of Mycoplasma mycoides subspecies mycoides small colony (MmmSC), the causative agent of Contagious Bovine Pleuropneumonia (CBPP), was compared in 15 mixed-breed Bos taurus steers. Two of these strains have not previously been investigated under experimental conditions. The cattle were inoculated endobronchiall...

Acrylamide gel electrophoresis was used to show the similarities and differences in the membrane proteins of two vaccine and two virulent strains of Mycoplasma mycoides var. mycoides. Immunoelectrophoretic (IEP) analysis was also used to partially characterize the associated antigens. Antibody spectra to the antigens of M. mycoides differ in rabbit, pig, and cattle sera. Rabbits produce better ...

Continuing our investigation of the tRNA genes and gene products in Mycoplasma mycoides, we report the sequence of the gene for tRNALeu (CAA) as well as partial primary structures of the following tRNAs: Leu (CAA), Leu (UAG), Arg (UCU), Thr (AGU) and Ile (CAU). It is suggested that in M. mycoides, at least some of the family codon boxes are read by only one tRNA each, using an unconventional me...

The detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (PCR) based methods. Although PCR can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. For this purpose, we tested the ability of flow cytometry (FC)...

i n a survey of pneumonia due to mycoplasma 282 out of 12168 ovine and caprine lung condemnation were collected (2.32%). mycoplasma spp. has been isolated from pneumonic cases in 4 sheep and 2 goats. pcr studies were confirmed the genus of mycoplasma although attempting for identification of strains m. mycoides, m. capricolum/caprine pleuropneumonia and m. arginin were in failure. the lesions i...

ba ck gr ou nd CBPP is a severe respiratory disease of cattle and buffalo caused by Mycoplasma mycoides subsp. mycoides biotype Small Colony (MmmSC). The agent of CBPP was recently isolated from goats at different times and places, even in areas free of CBPP. Thereby, goats should be considered a putative MmmSC reservoir. No test has been proposed so far for surveillance of CBPP in goats. Furth...