TORRETTI, JORGE, ERNESTO HENDLER, EDWARD WEINSTEIN, ROBERT E. LONGNECKER, AND FRANKLIN H. EPSTEIN. Functional sign@ance of NaK-A Vase in the kidney: e$ect.s of ouabain inhibition. Am. J. Physiol. 222(6): 1398-1405. 1972.-Infusions of ouabain directly into the renal artery of the dog produced substantial inhibition of renal Na-K-ATPase activity, which was almost complete in some experiments. The...

Disturbances of Na,K-ATPase activity are implicated in the pathophysiology of cerebral ischemia. Previous experiments have shown that EGb 761 protects NaK-ATPase activity against one hour of cerebral ischemia. In the brain however, the 3 isoenzymes responsible for Na,K-ATPase activity may be differentially affected by various times of ischemia. In the present study, we investigated the effect o...

We report on the creation of an ultracold dipolar gas of fermionic NaK molecules in their absolute rovibrational and hyperfine ground state. Starting from weakly bound Feshbach molecules, we demonstrate hyperfine resolved two-photon transfer into the singlet XΣ|v=0, J=0〉 ground state, coherently bridging a binding energy difference of 0.65 eV via stimulated rapid adiabatic passage. The spin-pol...

PURPOSE To localize NaK ATPase sites on cultured human retinal pigment epithelium (RPE). METHODS Cultured human RPE from fetal, 2-year-old, and 21-year-old donors was grown to confluence in microporous culture wells for 4 months to 2 years, mounted in a small-volume Ussing chamber, and perfused with growth medium. Ouabain (10(-5)-M) was applied to the basal and apical sides of the RPE. Change...

We examined immunohistochemically the localization of three transport enzymes (carbonic anhydrase, Ca-II; sodium-potassium-activated adenosine triphosphatase, NaK-ATPase; bicarbonate-chloride exchanger, band III) in the anterior segment of the human eye. In accord with earlier studies, NaK-ATPase was primarily found in the corneal endothelium, but also in the corneal basal epithelial cell membr...

Previous studies have suggested that an alteration in the expression of the NaK-ATPase of muscle may be an important determinant ofenhanced insulin sensitivity in chronic renal failure. Therefore, in the present studies we have examined the effect of uremia on the NaK-ATPase a isoforms in skeletal muscle, at the level of mRNA expression and enzymatic activity. The activity of the sodium pump, a...

The phosphotyrosine-binding (PTB) domain of the cell fate determinant Numb is involved in the formation of multiple protein complexes in vivo and can bind a diverse array of peptide sequences in vitro. To investigate the structural basis for the promiscuous nature of this protein module, we have determined its solution structure by NMR in a complex with a peptide containing an NMSF sequence der...

The electronic structure and the spectroscopic constants of the low lying electronic states of the NaK ionic molecule have been determined through using an ab initio approach involving a non-empirical pseudopotential for the Na and K cores and core valence correlation correction. The potential energy of nearly 26 electronic states of R, P, and D symmetries has been calculated up to their dissoc...