There are tow major methods that are widely used for protein identification by mass spectrometry: MALDI-TOF based protein fingerprinting and LC-MS/MS based peptide sequencing. In the MALDI-TOF based protein fingerprinting method, a sample is digested with certain proteolytic enzyme (usually trypsin) and one MS spectrum is acquired which generates the massed of all peptides (or MH+), and these m...

A novel monolithic enzymatic microreactor was prepared in the fused-silica capillary by in situ polymerization of acrylamide (AA), N-acryloxysuccinimide (NAS) and ethylene dimethacrylate (EDMA) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, which could offer very low back pressure, enabling the fast digestion of proteins. The performance of the monolithic microreac...

As a tribute to the scientific work of Professor Gareth Thomas in the field of structure-property relationships this paper delineates a new possibility of Lorentz transmission electron microscopy (LTEM) to study the magnetic properties of soft magnetic films. We show that in contrast to the traditional point of view, not only does the direction of the magnetization vector in nano-crystalline fi...

In this study, an integrated micro-nanofluidic system for protein analysis was presented. The device is comprised of a micromixer and a preconcentrator with a separation column. The Integrated micromixer based on unbalanced split and cross collision of fluid streams is passive and planar, which is easy to fabricate and integrate to the microfluidic system. The preconcentrator has nanochannels f...

This paper describes the synthesis and chromatographic and morphologic characterization of two monolithic silica nano-columns (50 µm i.d.) prepared by sol-gel processes, using hydrophilic interaction (HILIC) mode separations to evaluate their performance. Two types of monoliths were prepared by varying the precursors (tetraethoxysilane or a tetraethoxysilane-methyltrimethoxysilane mixture) and ...

Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-T...

A SRAM cell must meet requirements for operation in submicron/nano ranges. The scaling of CMOS technology has significant impact on SRAM cell -random fluctuation of electrical characteristics and substantial leakage current. In this paper we present dynamic column based power supply 8T SRAM cell and comparing the proposed SRAM cell with respect to conventional SRAM 6T in various aspects. To ver...

In this research, sensing performance of mono and bimetallic nanophotonics SERS sensors gold-silver nano-columns for the detection chlorpyrifos was investigated. For optimum substrates Gold-silver/nano-column surface-enhanced, Raman scattering (SERS) achieved with silicon substrate. By combining Ag activity Au chemical stability Si large field enhancement, Au-Ag/nano-columns substrate revealed ...