The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27oC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regen...

Explants were isolated from 6-day-old etiolated bean seedlings (Phaseolus vulgaris L. cv. Black Valentine) containing the cotyledons with 4 mm of hypocotyl just below the node and/or the epicotyl. During incubation on distilled water, uneven growth of the hypocotyl or epicotyl occurred resulting in the formation of a hook. The more rapid growth of the side which became convex was not dependent ...

Responses to auxin of Lotus corniculatus root tips or protoplasts transformed by Agrobacterium rhizogenes strains 15834 and 8196 were compared to those of their normal counterparts. Three different types of experiments were performed, involving long-term, medium-term, or short-term responses to a synthetic auxin, 1-naphthaleneacetic acid. Root tip elongation, proton excretion by root tips, and ...

The binding of alpha-naphthaleneacetic acid ((14)C-NAA) to pelletable particulates from corn (Zea mays) coleoptiles was found to be influenced by inorganic solutes. La(3+), Ca(2+), and Mg(2+) increased the binding whereas monovalent cations did not. The concentrations of CaCl(2) which increased auxin binding were similar to those which inhibited coleoptile elongation in the presence of auxin. T...

This article highlights the efficacy of plant growth regulators and nutrient solutions on root development and survival of ascending purple milkvetch (APMV) cuttings. Three auxin-like growth regulators and Hoagland solutions were used as root inducing agents. The cuttings were planted in sand medium that allowed evaluation of rooting effectiveness. The study showed that up to 100% establishment...

Abstract The presented study analyses the influence of salicylic acid (SA) and synthetic auxin 1-naphthaleneacetic (NAA) on total tanshinone level dihydrotanshinone (DHT), cryptotanshinone (CT), tanshinine I (TI) IIA (TIIA) in Salvia miltiorrhiza callus cultures growing solid Murashige Skoog (MS) medium. SA NAA was evaluated at 10-day intervals throughout a 80-day treatment period. applied 0.1,...

In this study, callus of P. tunicoides was used to induce adventitious roots. It was found that the medium with indole-3-butyric acid (IBA) was most suitable for induction of P. tunicoides adventitious roots from callus. To investigate the effects of concentration and type of auxins on adventitious roots induction, an orthogonal test was deployed. It was shown that 2,4Dichlorophenoxyacetic acid...

In response to iron (Fe) deficiency, dicots employ a reduction-based mechanism by inducing ferric-chelate reductase (FCR) at the root plasma membrane to enhance Fe uptake. However, the signal pathway leading to FCR induction is still unclear. Here, we found that the Fe-deficiency-induced increase of auxin and nitric oxide (NO) levels in wild-type Arabidopsis (Arabidopsis thaliana) was accompani...

The mulberry tree (Morus alba) is a perennial and fast-growing distributed worldwide under different climatic conditions. Most of the world’s silk production (>90%) facilitated by feeding silkworm larvae on leaves alba L.) varieties. Therefore, exploration protocol for improving propagation efficiency increasing reproductive capacity M. varieties could be great significance. This study aimed...

The role of alpha-naphthaleneacetic acid (NAA) in the control of abscission in Citrus (Citrus sinensis L. Osbeck) leaf explants and its conjugation were studied in non-aged and 24-hour-aged explants. Dipping non-aged explants in 1.5 micromolar NAA for 15 minutes immediately after excision did not delay abscission whereas 150 micromolar NAA effectively delayed it. As incubation time was prolonge...