The costs and benefits of polyandry are central to understanding the near-ubiquity of female multiple mating. Here, we present evidence of a novel cost of polyandry: disrupted sex allocation. In Nasonia vitripennis, a species that is monandrous in the wild but engages in polyandry under laboratory culture conditions, sexual harassment during oviposition results in increased production of sons u...

Female Nasonia vitripennis lay fewer eggs and increase the proportion of male offspring when ovipositing in previously parasitized hosts compared to unparasitized hosts. This study examines the location and nature of the cues that females use in these clutch size and sex ratio decisions. Neither the sex ratio nor the clutch size response relies on chemical cues on, or a hole drilled in, the out...

Activity rhythms in 24 h light-dark cycles, constant darkness, and constant light conditions were analyzed in four different Nasonia species for each sex separately. Besides similarities, clear differences are evident among and within Nasonia species as well as between sexes. In all species, activity in a light-dark cycle is concentrated in the photophase, typical for diurnal organisms. Contrar...

All insects in the order Hymenoptera have haplodiploid sex determination, in which males emerge from haploid unfertilized eggs and females are diploid. Sex determination in the honeybee Apis mellifera is controlled by the complementary sex determination (csd) locus, but the mechanisms controlling sex determination in other Hymenoptera without csd are unknown. We identified the sex-determination...

Empirical studies of how constrained females affect sex ratio are few. Constrained females are those that can produce only sons (e.g., in haplodiploid species, females that have not mated or older females that have used up their sperm). In the parasitoid wasp Nasonia vitripennis (Walker, 1836), failure to mate soon after emergence increased the probability of a female being constrained and thus...

Host preference is an important behaviour in parasitoid females as they must choose suitable hosts for their offspring. Preference includes location, recognition and acceptance of hosts. Both genetic environmental factors can affect all those stages. Here, we focus on two (rearing history experience) affecting host the wasp genus Nasonia (Hymenoptera: Chalcidoidea: Pteromalidae: Pteromalinae), ...

The transcription factor Fruitless exerts a broad range of functions during Drosophila development, the most apparent of which is the determination of sexual behavior in males. Although fruitless sequences are found in other insect orders, little is known about fruitless structure and function outside Diptera. We have performed a thorough analysis of fruitless transcripts in the haplo-diploid w...

Sex ratio theory provides a clear and simple way to test if nonsocial haplodiploid wasps can discriminate between kin and nonkin. Specifically, if females can discriminate siblings from nonrelatives, then they are expected to produce a higher proportion of daughters if they mate with a sibling. This prediction arises because in haplodiploids, inbreeding (sib-mating) causes a mother to be relati...

The foraging behaviour of a parasitoid insect species includes the host's habitat and subsequent location of the host. Habitats substrate, substrate moisture, and light levels can affect the host searching of different species of parasitoids. However, the depth at which parasitoids concentrate their search effort is another important ecological characteristic and plays an important role in loca...