A modified nitroblue tetrazolium test (NBT) is described which is suitable for routine application in the haematology or bacteriology laboratory and which provides a rapid aid to the diagnosis of bacterial infection. Hitherto published methods have recommended the use of heparinized whole blood samples for the performance of the NBT test. However, we have demonstrated that the use of the sucros...

1 . Washed buffy-coat preparations of human blood suspended in different media were exposed to the Nitroblue Tetrazolium (NBT) and the mechanism of entry of the dye into neutrophil polymorphonuclear leucocytes was investigated. 2. Stimulation of neutrophils with endotoxin and the presence of heparin and/or fibrinogen in the suspending medium were necessary for dye reduction. 3. NBT complexed wi...

The leukocytes of patients with chronic granulomatous disease (CGD) may be identified by their failure to reduce Nitro Blue Tetrazolium (NBT) during phagocytosis. This reaction, normally detected in the phagocytic vacuole, is absent or delayed in CGD monocytes and eosinophils as well as in neutrophils, even though sonicates of normal and CGD leukocytes contain equal activities of a cyanide inse...

The percentage nitroblue-tetrazolium (NBT) leucocyte levels were evaluated daily in the 10 days following a first attack of coronary thrombosis. In 12 uncomplicated cases the peak increase was on the second or third day; thereafter the level declined and was below 10% by the tenth day. Three other patients did not conform to this pattern of NBT behaviour. They had experienced a second myocardia...

Water-enhanced degradation of p-type low temperature polycrystalline silicon thin film transistors under negative bias temperature (NBT) condition is studied. H2O penetration into gate oxide network and the role of H2O during NBT stress are confirmed and clarified respectively. To prevent H2O diffusion, a combination of a layer of PECVD SiO2 and a layer of PECVD Si3N4 as passivation layers are ...

In this study, the humidity detection properties of ceramic with composition (Na0.5Bi0.5)0.94Ba0.06TiO3, noted (NBT-06BT), were investigated. The NBT-06BT was synthesized by semi-solid method and characterized X-ray diffraction (XRD) scanning electron microscopy (SEM). NBT-BT material sintered at 1100?C, crystallizes in tetragonal system without any secondary phase presents a heterogeneous dist...

Sodium–bismuth–titanate (NBT) has recently been shown to contain high levels of oxide ion conductivity. Here we report the effect A-site monovalent ions, M+ = K+ and Li+, on electrical conductivity NBT. The partial replacement Bi3+ with ions improved ionic by over one order magnitude without an apparent change conduction mechanism, which is attributed increase in oxygen vacancy concentration ba...

The quassinoid analogue NBT-272 has been reported to inhibit MYC, thus warranting a further effort 7to better understand its preclinical properties in models of embryonal tumors (ET), a family of childhood malignancies sharing relevant biological and genetic features such as deregulated expression of MYC oncogenes. In our study, NBT-272 displayed a strong antiproliferative activity in vitro tha...

The most common X-linked recessive form of chronic granulomatous disease (X-CGD) is characterized by the absence of cytochrome b558 in neutrophils. In a rare variant form of X-CGD, cytochrome b558 is present but not functional. The gene (locus symbol CYBB) was localized to band Xp21 by studies of patients with small chromosome deletions. The gene was cloned based on its location and found to en...

The quassinoid analogue NBT-272 has been reported to inhibit MYC, thus warranting a further effort 7to better understand its preclinical properties in models of embryonal tumors (ET), a family of childhood malignancies sharing relevant biological and genetic features such as deregulated expression of MYC oncogenes. In our study, NBT-272 displayed a strong antiproliferative activity in vitro tha...