Mutations in OPTN are associated with glaucoma, an eye disease, and also amyotrophic lateral sclerosis (ALS), a motor neuron disease. A 2-bp insertion (691_692insAG or 2bpIns-OPTN) is both glaucoma ALS. This mutation results frame shift after 127 amino acids, giving rise to protein C-terminal aberrant sequence. We have explored the mechanism of induction cell death by this mutant line, NSC-34, ...

Spinal cord injury (SCI) is a common and potentially life-threatening condition with no established treatment to treat the primary injury. Mesenteric neural stem cell (NSC) therapy promising SCI in chronic phase. We aimed review literature narratively describe current evidence regarding mesenteric NSC SCI. Primary refers tissue damage that occurs at time of trauma leads death neuronal cells. In...

Nondeterministic analogues of the well-known classes NC and SC, d d NNC and NSC, respectively, are investigated. Adding nondeterminism to SC leaves it in the domain of parallel computation since NSC E POLYLOGSPACE. That is, NSC is a subset of the class of languages computable by fast parallel computers. Adding noodeterminism to NC appears to make it much more powerful since NNC = NP. It is clea...

Hypoxia-induced pulmonary vasoconstriction (HPV) is critical for matching of ventilation/perfusion in lungs. Although hypoxic inhibition of K(+) channels has been a leading hypothesis for depolarization of pulmonary arterial smooth muscle cells (PASMCs) under hypoxia, pharmacological inhibition of K(+) channels does not induce significant contraction in rat pulmonary arteries. Because a partial...

Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC), to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effects of NSC extractum was relatively weak and similar on C. gloeosporioides Penzig and P. italicum....

The present study aimed to investigate neural stem cell (NSC)-specific regulatory promoter elements. PCR was employed to amplify the full sequence (4,000 bp) and core sequence (400 bp) of the promoter and intron-2 of the mouse nestin gene. pcDNA3.1 was used as a template to construct 6 different recombinant plasmids. CMV, CMV + intron-2, the full sequence of the nestin gene promoter, the full s...

The US military has placed major emphasis on developing therapeutics against nerve agents (NA). Current efforts are hindered by the lack of effective in vitro cellular models to aid in the preliminary screening of potential candidate drugs/antidotes. The development of an in vitro cellular model to aid in discovering new NA therapeutics would be highly beneficial. In this regard, we have examin...

The effects of oxidized low-density lipoprotein (OxLDL) and its major lipid constituent lysophosphatidylcholine (LPC) on Ca(2+) entry were investigated in cultured human umbilical endothelial cells (HUVECs) using fura-2 fluorescence and patch-clamp methods. OxLDL or LPC increased intracellular Ca(2+) concentration ([Ca(2+)](i)), and the increase of [Ca(2+)](i) by OxLDL or by LPC was inhibited b...

Recent studies have explored the potential of central nervous system-derived neural stem cells (CNS-NSC) to repopulate the enteric nervous system. However, the exact phenotypic fate of gut-transplanted CNS-NSC has not been characterized. The aim of this study was to investigate the effect of the gut microenvironment on phenotypic fate of CNS-NSC in vitro. With the use of Transwell culture, diff...