Contents 1 Introduction 1 1.1 Protein-protein-interaction screenings in vivo 2 1.1.1 Yeast two-hybrid screens 2 1.1.2 in vivo pulldown assays 4 1.1.3 Data integration and critical evaluation 6 1.2 Protein-protein-interaction screenings in vitro 6 1.2.1 Protein arrays 6 1.2.1.1 Protein microarrays 7 1.2.1.2 Arrayed cDNA expression libraries 9 1.2.2 Peptide arrays 10 1.3 Other in vitro techniques...

The zebrafish is a vertebrate model compatible with the paradigms of drug discovery. The small size and transparency of zebrafish embryos make them amenable for the automation necessary in high-throughput screenings. We have developed an automated high-throughput platform for in vivo chemical screenings on zebrafish embryos that includes automated methods for embryo dispensation, compound deliv...

Bedside hearing screenings are routinely conducted by speech and language pathologists for brain injury survivors during rehabilitation. Cognitive deficits resulting from brain injury, however, may interfere with obtaining estimates of auditory thresholds. Poor comprehension or attention deficits often compromise patient abilities to follow procedural instructions. This article describes the ef...

In the last years high-throughput pharmacophore screenings have been rediscovered as an effective and rapid tool for guiding the selection of new hit compounds with predefined biological activity. This renaissance has also been fostered by the current possibility to generate pharmacophore hypotheses directly from crystallographic, NMR or computational models of proteinligand complexes [1,2]. In...

Abstract: Between 1992 and 2009, 30 US states adopted laws mandating that health insurance plans cover screenings for prostate cancer. Because prostate cancer screenings are used almost exclusively by men over age 50, these mandates raise the cost of insuring older men relative to other groups. This paper uses a triple-difference empirical strategy to take advantage of this quasi-random natural...

Distortion product otoacoustic emissions (DPOAE) are sensitive to both sensorineural and conductive hearing losses and have the potential to be used as an effective screening measure across all populations, including children. DPOAE offer a quick and straightforward hearing screening technique for the pediatric population that is not infl uenced by subjective testing and is highly reproducible....

Human skin equivalents (HSEs) can be considered a valuable tool to study aspects of human skin, including the skin barrier, or to perform chemical or toxicological screenings. HSEs are three-dimensional skin models that are usually established using primary keratinocytes and closely mimic human skin. The use of primary keratinocytes has several drawbacks, including a limited in vitro life span ...

Small or shrivelled wheat kernels (screenings) that reduce crop value are commonly produced in terminal drought environments. The aim of this study was to establish whether the incorporation of the tiller inhibition (tin) gene would contribute to maintenance of kernel weight and reductions in screenings under terminal water deficit. Five Silverstar near-isogenic lines contrasting in high and lo...

OBJECTIVE To determine the associations between personality subscales and attendance at gastric cancer screenings in Japan. METHODS A total of 21,911 residents in rural Japan who completed a short form of the Eysenck Personality Questionnaire-Revised (EPQ-R) and a questionnaire on various health habits including the number of gastric cancer screenings attended were included in the primary ana...

Weaned bison bull calves were randomly assigned to four feedlot diets in a 4 treatment by 4 periods Latin square study. The feeding periods were 80 days long during spring, summer, fall, and winter. The four dietary treatments were identified based on major ingredients in each ration: 1) wheat screenings, 2) wheat middlings, 3) crambe meal, and 4) a commercial bison ration. Winter gains were lo...