نتایج جستجو برای: nested polymerase chain reaction
تعداد نتایج: 707924 فیلتر نتایج به سال:
Magellanic penguins (Spheniscus magellanicus) are native to Argentina, Chile and the Falkland Islands. Magellanic penguins are highly susceptible to blood parasites such as the mosquito-borne Plasmodium spp., which have been documented causing high morbidity and mortality in zoos and rehabilitation centres. However, to date no blood parasites have been detected in wild Magellanic penguins, and ...
OBJECTIVE To determine the prevalence of the Torque teno virus in healthy donors in the northern and northwestern regions of the state of Paraná, southern Brazil. METHODS The Torque teno virus was detected by a nested polymerase chain reaction using a set of oligoprimers for the N22 region. RESULTS The prevalence of the virus was 69% in 551 healthy blood donors in southern Brazil. There was...
Using nested polymerase chain reaction, we sequenced Dobrava virus (DOB) from the rodent Apodemus agrarius in Hungary. The samples we isolated group with DOB samples previously isolated from A. flavicollis. This grouping may indicate host switching.
results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...
بیماری¬های ویروسی ناشی از نپوویروس¬ها از مخرب¬ترین بیماری¬های ویروسی مو در سراسر جهان به حساب می¬آیند که با نماتدهای خانواده longidoridae منتقل می¬شوند. اغلب این ویروس¬ها دارای میزبان¬های طبیعی متعددی در بین گیاهان زینتی و غیر زینتی، درختان میوه و علف¬های هرز می¬باشند. پیکره اعضای این جنس دو بخشی، جورقطر و به قطر حدود 30 نانومتر می¬باشد. غلظت این ویروس¬ها در گیاه و به ویژه تاک پایین است و در نت...
background aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). objectives this study aimed to evaluate the frequency of aseptic meningitis caused by herpesviruses, namely herpes simplex types i and ii (hsv-1, hsv-2), epstein-barr virus (ebv), cytomegalovirus (...
conclusions in the present study, the highest prevalence was for st5, which is so important for epidemiology and risk of human infection. the report related to st3 in cattle as a subtype of human showed mutual infection between human and cattle. another important point in this study was the st6 report. finally, it seems that gathering epidemiological data is needed for a better understanding of...
background toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. the parasite is able to infect all warm-blooded hosts including human.the infection occures via consumption of food or water containing oocytes, eatingundercooked meats containing tissue cysts, and placenta. undercooked meat consumption is one of the most important ways of toxoplasma transm...
conclusions using the pcr-rflp method, three distinctive h. pylori caga genotypes were detected in antral biopsies. genotype i, which was predominant among the isolates, was significantly associated with gastritis. however, the data showed that caga genotype iii may play a role in duodenitis and duodenal ulcers in patients infected with h. pylori. patients and methods a total of 161 gastric bio...
background human t-lymphotropic virus type i (htlv-i) is one of the health threatening problems in endemic areas and can be transmitted by different routes such as blood transfusion. in order to correct chronic anemia in thalassemia subjects, they may need to get blood units per month. thus, they are at risk of infection with blood-borne viruses such as htlv-i. objectives in the present study, ...
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