BACKGROUND AND OBJECTIVES The properties of neuraminidase produced by P. aeruginosa strain PAO1 during growth in a defined medium (BHI) was examined and compared with some neuraminidase features of K. pneumoniae in this investigation. MATERIALS AND METHODS The enzyme was isolated from concentrated culture supernatants of P. aeruginosa which was used in a sensitive fluorometric assay by using ...

The structure of the influenza virus neuraminidases, the spatial organization of their active site, the mechanism of carbohydrate chains desialylation by neuraminidase, and its role in the influenza virus function at different stages of the viral infectious cycle are considered in this review. Data on the neuraminidase substrate specificity and different approaches in studying the activity of t...

Phospholipid nanogels enhance the stability and performance of the exoglycosidase enzyme neuraminidase and are used to create a fixed zone of enzyme within a capillary. With nanogels, there is no need to covalently immobilize the enzyme, as it is physically constrained. This enables rapid quantification of Michaelis-Menten constants (KM) for different substrates and ultimately provides a means ...

The design principle of a biochemiluminescent instrument is presented. diagnoses influenza based on detection the flu viral neuraminidase activity. uses photomultiplier tube (PMT) as sensor, with 24 test channels. reaction disk driven by step motor to rotate, where reagent incubated. Finally, PMT detects number photons emitted and calculates enzyme correlation coefficient (R2) 2.83 5.06 log TCI...

Alkaline phosphatase from human liver was purified to homogeneity. The purification procedure included solubilization with butanol, fractionation with acetone, and chromatography on concanavalin A-Sepharose, DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex. Purity was established by standard and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The isoelectric point of the protein was...

Genetic recombination of influenza viruses of different subtype may result in segregation of hemagglutinin and neuraminidase in antigenically hybrid viruses [E. D. Kilbourne et al., in M. Pollard (ed.), Perspectives in Virology, vol. 5, p. 87, Academic Press, Inc., N.Y., 1967; and W. G. Laver and E. D. Kilbourne, Virology 30:493, 1966]. The selective use of such antigenic hybrids in serological...

Nine of the twelve known variants of human transferrin have been resolved by the action of neuraminidase into stepwise patterns of four additional slower moving components whose relative intensities depended upon the concentration of enzyme. These components appeared to represent the stepwise removal of the four sialic acid residues from the transferrin molecule, and at large enzyme concentrati...

Influenza A viruses possesses two virion surface glycoproteins including haemagglutinin (HA) and neuraminidase (NA). The NA plays an important role in viral replication and promotes virus release from infected cells and facilitates virus spread throughout the body. To find out any genomic changes that might be occurred on NA gene of avian influenza circulating viruses, we have genetically analy...

In a recent study, we found neuraminidase activity to be present in only 7 of 15 cultures of various types and strains of laboratory-adapted Diplococcus pneumoniae (R. T. Kelly, D. Greiff, and S. Farmer, J. Bacteriol. 91:601, 1966). To determine whether a correlation existed between the presence of neuraminidase activity in pneumococci and human pneumococcal illness, we have studied freshly iso...

The monoclonal antibody AGF4.48 detects the 3-fucosyl-N-acetyllactosamine carbohydrate structure and reacts with a variety of human tissues, as shown by an immunoperoxidase method on paraffin sections of formalin fixed material. The intensity and distribution of the immunoperoxidase reactivity were affected by treatment of sections with neuraminidase. In some sites, such as bone marrow, brain, ...