In antimalarial research there are no standard procedures to determine the toxicity of a drug candidate. Among alternatives available, in vitro cytotoxicity assays most widely used predict toxic effects future therapeutic products. They have advantage over vivo assays, that they offer possibility restrain number experimental variables. The objective present study was compare cytotoxic methods b...

The use of lysosomal stability in the mussel, Mytilus galloprovincialis, as a potential biomarker of environmental contamination has been evaluated along the Portuguese coast. To this end, the neutral red retention (NRR) time was measured in mussel haemocytes gathered from nine different locations reflecting different degrees of anthropogenic contamination. Mussels collected in the vicinity of ...

In this research we have developed a treatment method for textile wastewater by TiO2/SiO2-based magnetic nanocomposite. Textile wastewater includes a large variety of dyes and chemicals and needs treatments. This manuscript presents a facile method for removing dyes from the textile wastewater by using TiO2/SiO2-based nanocomposite (Fe3O<su...

Neutral Red (NR), a eurhodin dye, is often used for staining living cells, but we demonstrated the first time that NR can also serve as CO2 sensor, because of NR’s unique optical properties, which change with dissolved carbon dioxide (dCO2) concentrations. In present study, sensitivity was quantified function changes in absorption and emission spectra to dCO2 pH 7.3 buffer medium at eight exhib...

Background: In recent years, there has been a growing interest in the development of sub-lethal earthworm biomarkers as they are relevant indicators of environmental change and they are among the five key indicators for ecotoxicological testing of industrial chemicals determined by the OECD. In the present study, the effects of an organophosphate pesticide dichlorovos on lysosomes of coelomoc...

A simple method to immobilize poly(neutral red) (PNR) and flavin adenine dinucleotide (FAD) hybrid film (PNR/FAD) by cyclic voltammetry is proposed. The PNR/FAD hybrid film can be easily prepared on an electrode surface involving electropolymerization of neutral red (NR) monomers and the electrostatic interaction between the positively charged PNR and the negatively charged FAD. It exhibits ele...

Naran R is a herbal composition made of Plantago lanceolate folium, Malvae arboreae flos, Calendulae flos, Chamomillae inflorescentia, Lamii albi flos to prepare compresses or to wash skin with inflammations. The extract of this preparation is mixed to be applied as an ointment on patients' skin after radiotherapy. Experiments performed in vitro are part of pre-clinical tests with Naran R ointm...

Synthetic organic nanotubes self-assembled from bolaamphiphile surfactants are now being explored for use as drug delivery vehicles. In this work, several factors important to their implementation in drug delivery are explored. All experiments are performed with the nanotubes immersed in ethanol. First, Nile Red (NR) and a hydroxylated Nile Red derivative (NR-OH) are loaded into the nanotubes a...

These days, environmental pollution, notably water has increasingly caused severe human health problems. The major pollutants are heavy metals. MnFe2O4/GO nanocomposite was prepared in the current work via situ method and tested to remove lead ion Pb2+ neutral red (NR) dye from water. characterized using different techniques, including X-ray diffraction, transmission electron microscopy, Fourie...