To determine if nitration of tyrosine residues by peroxynitrite (PN), which can be generated endogenously, can disrupt the phosphorylation of tyrosine residues in proteins involved in cell signaling networks, we studied the effect of PN-promoted nitration of tyrosine residues in a pentadecameric peptide, cdc2(6-20)NH2, on the ability of the peptide to be phosphorylated. cdc2(6-20)NH2 correspond...

Background: Nitrase Therapeutics is unlocking the therapeutic potential of nitrases, a new class enzymes discovered by company that catalyze post-translation modification called nitration. Materials And Methods: Nitrases nitration, process which an NO2 group transferred from peroxynitrite to specific tyrosines on substrates (nitro-substrates). Nitration important modifier protein structure, fun...

Nitration of p-tert-butylcalix[n]arene methyl ethers under a variety of reaction conditions has been examined. It has been determined that amongst different nitration procedures adopted (AlCl3/KNO3, HNO3/CH3COOH, HNO3/(CH3CO)2O, cerium(IV) ammonium nitrate/CH3COOH), ipso-nitration with CH3COOH/HNO3 gives best yields of p-nitrocalixarenes and work up conditions. ipso-Nitration of tetramethoxytet...

In recent years, the study of nitric oxide (NO) in plant systems has attracted the attention of many researchers. A growing number of investigations have shown the significance of NO as a signal molecule or as a molecule involved in the response against (a)biotic processes. NO can be responsible of the post-translational modifications (NO-PTM) of target proteins by mechanisms such as the nitrat...

Nitration of pollen derived allergens can occur by NO(2) and ozone in polluted air and it has already been shown that nitrated major birch (Betula verrucosa) pollen allergen Bet v 1.0101 (Bet v 1) exhibits an increased potency to trigger an immune response. However, the mechanisms by which nitration might contribute to the induction of allergy are still unknown. In this study, we assessed the e...

Aulak, Kulwant S., Thomas Koeck, John W. Crabb, and Dennis J. Stuehr. Dynamics of protein nitration in cells and mitochondria. Am J Physiol Heart Circ Physiol 286: H30–H38, 2004; 10.1152/ ajpheart.00743.2003.—Nitric oxide is a precursor of reactive nitrating species such as peroxynitrite and nitrogen dioxide that modify proteins to generate 3-nitrotyrosine. Many diseases are associated with inc...

Aromatic nitration has been used to modify the polymer mam chain of polysulfone and polyethersulfone Alteration m the gas separation charactenstlcs of homogeneous membranes made out of these polymers could be observed upon nitration Generally, separation factors for gas mixtures of CO&H, increased while the permeability decreased Remarkably, a decreasing dlffuslvlty for both gases upon nitratio...

Nitro catechols via 2 hydroxyl group and a nitro group have a wide range of functions including reagents form stable complexes with more than 40 elements in the fields of analytical chemistry, biochemistry, coordination chemistry, environmental chemistry, atrium exploration, organic chemistry, pharmacy, etc. Moreover, the nitro to amine recovery has a potential function in different fields. In ...

When iron . lactoferrin and apolactoferrin were submitted to nitration with tetranitromethane, the number of unmodified tyrosine residues recovered upon acid hydrolysis was 4-5 in the former case against 0-1 in the latter. The iron-binding capacity of lactoferrin appeared to remain intact following nitration of the iron-containing form of the protein, whereas metal-combining properties were des...

Density-functional theory (DFT) calculations at the LC-wHPBE/6-311++G(d,p) level found that textbook three-step nitration mechanism of benzene in mixed acids was seriously wrong. Step 1 generating nitronium ion (NO2+) is not spontaneous, NO2+ generated by Lewis collision, and needs to overcome a barrier Ea = 18 or 22 kcal/mol acid nitric acid. Obtaining collision quantum chemical highlight stud...