We present results from laboratory investigations on the fluorescence of Dense NonAqueous Phase Liquids (DNAPL) and fluorophore mixtures, characterized using a Luminescence Spectrometer. Fluorophores such as aromatic compounds (e.g. benzene, toluene, ethylbenzene and xylenes, as well as other constituents of petroleum fuels, oils, lubricants and greases) are present as co-contaminants in many D...

This comprehensive paper, Electrode Potentials Part 2, is a continuation of 1: Fundamentals and Aqueous Systems. Determining the electrode potential crucial for understanding nature electrochemical properties materials or systems; however, an accurate evaluation target has always been challenge. The can be used to predict reaction mechanisms in electrochemistry directly applied study applicatio...

A number of application for enzymes in organic solvents have been developed in chemical processing, food related conversions and analyses. The only unsolved problem related to nonaqueous enzymology is the notion that enzymes in organic solvent are mostly far less active than in water. Therefore, studies concerning the mechanisms by which enzymes are inactivated by organic solvents would reveal ...

The complexation reaction between salen (Bis(salicylidene)ethylenediamine) and Cu(II) cations in methanol (MeOH), 2-propanol (2-PrOH), acetonitrile (AN), tetrahydrofuran (THF), and chloroform (CHCl3) as nonaqueous solvents at 25º C has been investigated. The stoichiometry and formation constants of complexes have been determined spectrophotometrically and conductometrically by method of continu...

Chloroethenes like tetrachloroethene (PCE) are the most prevalent groundwater contaminants in the USA. Their presence as nonaqueous phase liquids (NAPLs) makes remediation difficult. Among options for NAPL cleanup, co-solvent injection has demonstrated success. However, the process has the potential to leave considerable residue of the co-solvent as well as residual chloroethene. Our rationale ...

The enthalpy of hydrogen-bond formation between guanine (G) and cytusine (C) in o-dichlorobenzene and in chloroform at 25°C has been determined by direct calorimetric measurement. We derivatized 2’-deoxyguanosine and 2‘-deoxycytidine at the 5’and 3’-hydroxyls with triisopropylsilyl groups; these group increase the solubility of the nucleic acid bases in nonaqueous solvents. Such derivatization ...

The x-ray crystal structures of trans-cinnamoyl-subtilisin, an acyl-enzyme covalent intermediate of the serine protease subtilisin Carlsberg, have been determined to 2.2-A resolution in anhydrous acetonitrile and in water. The cinnamoyl-subtilisin structures are virtually identical in the two solvents. In addition, their enzyme portions are nearly indistinguishable from previously determined st...