For buildings that meet the requirements of current seismic design codes, damage to nonstructural components and internal objects often become main source economic losses these buildings. However, specifications only consider safety ‘no collapse under strong earthquake’ do not ‘functional recoverability’. In this paper, a six-story frame building was taken as an example. Four joint performance ...

An experimental investigation on the chemical, physical, mechanical, and shrinkage of seawater sea sand-based ultra-high-performance concrete (UHP-SWSSC) with supplementary cementitious materials (SCM) (i.e. slag silica fume) is reported. Several mixes were designed varying proportions SCMs (25%, 37.5%, 50%, 62.5% binder), aggregate source, water-to-binder ratio. Heat evolution, density, workab...

In order to increase our understanding of the assembly of the capsid proteins of AHSV, for the development of subunit vaccines and to study viral morphogenesis, it is necessary to investigate the interaction of the four major structural proteins of AHSV in the absence of other AHSV proteins and the viral genome. To investigate particle formation it was necessary to synthesise the proteins of in...

The Sindbis-group alphavirus S.A.AR86 encodes a threonine at nonstructural protein 1 (nsP1) 538 that is associated with neurovirulence in adult mice. Mutation of the nsP1 538 Thr to the consensus Ile found in nonneurovirulent Sindbis-group alphaviruses attenuates S.A.AR86 for adult mouse neurovirulence, while introduction of Thr at position 538 in a nonneurovirulent Sindbis virus background con...

Sindbis virus and Ross River virus are alphaviruses whose nonstructural proteins share 64% identity and whose structural proteins share 48% identity. Starting from full-length cDNA clones of both viruses, we have generated two reciprocal Sindbis-Ross River chimeric viruses in which the structural and nonstructural regions have been exchanged. These chimeric viruses replicate readily in several ...

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Plasmids were constructed which contained a large portion of each of the four nonstructural genes of Sindbis virus fused to the N-terminal two-thirds of the trpE gene of Escherichia coli. The large quantity of fusion protein induced from cells containing these plasmids was subsequently used as an antigen to generate polyclonal antisera in rabbits. Each antiserum was specific for the correspondi...

Abstract Most structural health monitoring systems estimate the overall behavior by measuring acceleration response, which cannot directly measure stress or damage state of individual members. An alternative approach is to use strain measurements; however, methods for analyzing and utilizing data actual steel buildings have not been established. In this study, highly precise semiconductor gauge...