Serum oestradiol and progesterone concentrations were examined for up to 7 days after withholding gonadotrophins whilst continuing pituitary down-regulation in 15 women at serious risk of severe ovarian hyperstimulation syndrome (OHSS) (serum oestradiol >6000 pg/ml and >15 follicles per ovary). Serum oestradiol concentrations rose on day 1 of coasting in all but two of the 15 women before falli...

INTRODUCTION Luteal phase support in assisted reproductive technology (ART) cycles is still controversial. The present study was conducted to evaluate the effect of adding oral oestradiol to progesterone during ART cycles. MATERIAL AND METHODS In this prospective case control study, infertile women under 35 years old who were candidates for IVF/ICSI cycles in Royan Institute were enrolled. A ...

Pulmonary hypertension continues to be a serious clinical problem with high mortality. As oestrogen is a potential vasodilator of the pulmonary circulation, this study examined the mechanisms by which 17β-oestradiol improves monocrotaline (MCT)-induced pulmonary hypertension. Female Sprague-Dawley rats underwent bilateral ovariectomy or sham operations. The rats received MCT (50 mg·kg(-1)) and ...

The effect of opioid peptides on cultured, oestradiol-stimulated human myometrial cells was examined. Oestradiol increased cell densities in mixed-cell (smooth muscle cells + stromal fibroblasts) cultures by 40%. This oestradiol-induced stimulation of cell proliferation was decreased to control values by D-met2-pro5-enkephalinamide. The half-effective inhibitory concentration of enkephalinamide...

Oestradiol-178 is the most active oestrogen in premenopausal women. Assays for oestradiol are of value in the investigation of normal and abnormal ovarian function. Rapid oestradiol measurements are effective in monitoring ovulation induction in the treatment of female infertility, and sensitive assays facilitate the detection of lower oestradiol concentrations found in men and postmenopausal w...

1. Ovariectomy-delayed blastocysts flushed from mice treated with medroxyprogesterone acetate were injected into the lumina of immature mouse uterine horns. The horns were then maintained as organ cultures for 1-3 days in media that contained either (a) ethanol or ethanol plus (6) progesterone, (c) oestradiol or (d) progesterone and oestradiol. 2. Trophoblast invasion of the uterine stroma occu...

BACKGROUND To discuss the meaning of serum oestradiol monitoring in frozen embryo transfer cycle using hormone replacement without pretreatment with gonadotropin hormone (GnRH) agonist. METHODS The data from two hundred twelve women undergoing two hundred seventy-four frozen-thawed embryo transfer (FET) cycles was included in this retrospective cohort study. They were detected of serum oestra...

In the previous studies a hypothesis was developed from the results that the increase in leptin concentrations during the second half of the menstrual cycle may be related to changes in the steroidal milieu during the preovulatory period and the luteal phase. The present study was undertaken to test this hypothesis further by examining the effect of treatment with oestradiol and progesterone on...

The activity of the oestradiol-17 beta hydroxysteroid dehydrogenase in human endometrial and breast cancer specimens was determined by the NAD-dependent conversion of oestradiol-17 beta to oestrone. The sensitivity of the determination was improved by the separation of the hormones by HPLC. We are now able to determine oestradiol-17 beta hydroxysteroid dehydrogenase quantitatively in cryostat s...

Tamoxifen is a potent inhibitor of specific oestrogen-induced yolk protein synthesis by chicken liver. The oestradiol receptor in salt extracts of liver nuclei from oestrogen-treated chicks has a K(d) for oestradiol of 0.7+/-0.2nm. Tamoxifen and its metabolite, monohydroxytamoxifen, compete for binding to the salt-soluble nuclear receptor with K(i) values of 2.6 and 0.1nm respectively. The anti...