A rapid identification assay for Waitea circinata (anamorph: Rhizoctonia spp.) varieties zeae and circinata causing patch diseases on turfgrasses was developed based on the universally primed PCR (UP-PCR) products cross-blot hybridization. Tester isolates belonging to the two varieties of W. circinata were amplified with a single UP primer L21, which generated multiple DNA fragments for each va...

Pathogenicity and anastomosis groups of seven Rhizoctonia-like isolates obtained from infected pistachio (Pistacia vera L.) seedlings were determined, in the central district Siirt province at Southeast Anatolia Region Turkey. Browning on crown root collected saplings drying plants observed. In pathogenicity test, most virulent isolate was Rs2 with a disease severity 93.75%. The group this isol...

Four hundred bacterial isolates were isolated from rhizosphere of some plants collected from Egypt and screened for production of chitinase enzyme. Only four isolates designated MS1, MS2, MS3 and MS4 were the most potent chitinolytic bacterial species. SDS-PAGE analysis of vegetative and sporulated cells of the four isolates revealed that the protein profile of the four isolates were different ...

Rhizoctonia solani is a causative agent of sheath blight, which results in huge economic losses every year. During its life cycle, the formation of sclerotia helps Rhizoctonia solani withstand a variety of unfavorable factors. Oxidative stress is a key factor that induces sclerotium formation. The differentiated and undifferentiated phenotypes of R. solani AG-1-IA were obtained by controlling a...

Rhizoctonia solani anastomosis group AG2-2 IIIB is a severe sugar beet and maize pathogen. It causes crown and root rot disease which le ads to y ield losses world-wide. The soil-borne pathogen is d ifficult to detec t and quantify by conventional methods. We developed a real-time PCR (qPCR) assay for the quantification of genomic DNA of Rhizoctonia solani AG2-2 IIIB based on the ITS region of ...