Two PCR primer pairs were designed to amplify rRNA genes (rDNA) from all four major phyla of fungi: Ascomycota, Basidiomycota, Chytridomycota, and Zygomycota. PCRs performed with these primers showed that both pairs amplify DNA from organisms representing the major taxonomic groups of fungi but not from nonfungal sources. To test the ability of the primers to amplify fungal rDNA from environmen...

Post-harvest fruit grading is a necessary step to avoid disease related loss in quality. In this paper, hierarchical method proposed (1) remove the background and (2) detect images that contains grape diseases(botrytis, oidium, acid rot). Satisfying segmentation performances were obtained by Lite Unet model with 92.9% IoU score an average speed of 0.16s/image. A pretrained MobileNet-V2 94% F1 o...