Multiple sclerosis is an inflammatory disease of the CNS leading to the destruction of oligodendrocytes (OLs), myelin sheaths and axons. The mediators of tissue injury remain unknown. Glutamate, which can be released by activated immune cells or produced within the CNS, has been implicated as a potential mediator of tissue injury in multiple sclerosis. alpha-Amino-3-hydroxy-5-methyl-4-isoxazole...

Death of oligodendrocyte (OL) precursors can be triggered in vitro by cystine deprivation, a form of oxidative stress that involves depletion of intracellular glutathione. We report here that OLs demonstrate maturation-dependent differences in survival when subjected to free radical-mediated injury induced by glutathione depletion. Using immunopanning to isolate rat preoligodendrocytes (preOLs)...

This study is motivated by the potential problem of using observational data to draw inferences about treatment outcomes when experimental data are not available. We compare two statistical approaches, ordinary least-squares (OLS) and instrumental variables (IV) regression analysis, to estimate the outcomes (three-year post-treatment survival) of three treatments for early stage breast cancer i...

Homoskedasticity is an important assumption in ordinary least squares (OLS) regression. Although the estimator of the regression parameters in OLS regression is unbiased when the homoskedasticity assumption is violated, the estimator of the covariance matrix of the parameter estimates can be biased and inconsistent under heteroskedasticity, which can produce significance tests and confidence in...

Recent studies suggest that ouabain or a ouabain-like substance (OLS) may be present endogenously in humans. We developed a RIA for ouabain with antisera raised in goat against ouabain conjugated to keyhole limpet hemocyanin and ovalbumin. The antiserum was of high antibody titer (200,000) and was specific for ouabain, with little cross-reactivity with common steroids and structurally related c...

Scarce access to primary samples and lack of efficient protocols to generate oligodendrocytes (OLs) from human pluripotent stem cells (hPSCs) are hampering our understanding of OL biology and the development of novel therapies. Here, we demonstrate that overexpression of the transcription factor SOX10 is sufficient to generate surface antigen O4-positive (O4+) and myelin basic protein-positive ...

This paper presents a two-level learning method for designing an optimal Radial Basis Function Network (RBFN) using Adaptive Velocity Update Relaxation Particle Swarm Optimization algorithm (AVURPSO) and Orthogonal Least Squares algorithm (OLS) called as OLS-AVURPSO method. The novelty is to develop an AVURPSO algorithm to form the hybrid OLS-AVURPSO method for designing an optimal RBFN. The pr...

We consider linear regression models where both input data (the values of independent variables) and output data (the observations of the dependent variable) are interval-censored. We introduce a possibilistic generalization of the least squares estimator, so called OLS-set for the interval model. This set captures the impact of the loss of information on the OLS estimator caused by interval ce...

State politics researchers commonly employ ordinary least squares (OLS) regression or one of its variants to test linear hypotheses. However, OLS is easily influenced by outliers and thus can produce misleading results when the error term distribution has heavy tails. Here we demonstrate that median regression (MR), an alternative to OLS that conditions the median of the dependent variable (rat...

Oligodendrocytes (OLs), the myelinating cells of the central nervous system, have specialized morphologies that subserve their function. Numerous qualitative studies suggest that OLs in different brain regions can differ in their morphological characteristics, including number of branches and internodes, internode length, etc. However, progress in identifying and characterizing the diverse type...