objective: enterotoxigenic escherichia coli is considered as the most important agent of children diarrhea and mortality in developing countries. this bacterium causes 300-600 thousands of deaths in the children under 5 years of age per year. with difficulties in treatment as well as its wide prevalence, designing an effective vaccine against this microorganism is the objective of world health ...

Background: Leukemia inhibitor factor (LIF) is a very important pleiotropic cytokine which belongs to interleukin-6 (IL-6) family. LIF exerts multiple effects on different types of cells and tissues with numerous regulatory effects in vivo and in vitro. It is a lymphoid factor, which performs a number of activities including cholinergic neuron differentia‌tion, contro...

conclusions over-expression of the synthetic cpc/β protein in the bacterial system (escherichia coli bl-21) showed that e. coli can be used as a basis for further research to produce this desired protein in large quantities. results the sds-page analysis and dot blotting confirmed the production of recombinant c-pc/β in the bacterial expression system. over-expression of cpcb gene was optimized...

Introduction: Helicobacter pylori (H. pylori) infection has remained as a global health problem. Animal studies demonstrated the role of H. pylori oipA gene in the development of gastric cancer. The aim of this study was the cloning and expression of Helicobacter pylori oipA gene in a bicistronic vector harboring mice IL-18 gene. Materials and methods: The target gene encoding oipA was amp...

Background: The increase of the protein expression via ribosomal manipulation is one of the suggested cellular mechanisms involved in EnBase fed-batch mode of cultivation. However, this system has not been implemented for cytotoxic proteins.Objectives: Here, the expression pattern of α-Luffin, a ribosome inactivation protein (RIP) with an innate toxicity,...

the alternative use of non-selective herbicides in different years will reduce the possibility of weeds resistance to herbicide by avoiding the crops constant exposure to herbicide selection pressure. the photoperiod-sensitive genic male sterile (pgms) rice with herbicide resistance will be convenient to the mechanization of hybrid seed production and weed control. in order to develop double he...

protein a is a commercially important protein in biotechnological and medicinal applications. the great value of this protein and its applications in genetic and protein engineering and microbial researches as well as the growing use in biochemical industries, biotechnology, medicine and pharmacology, highlight the importance of the present study. in this survey the encoding genes of full-lengt...

results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...