This paper proposes a GA-based reduced search space technique (GA-RSS) for the optimal design of steel moment frames. It tries to reduce the computation time by focusing the search around the boundaries of the constraints, using a ranking-based constraint handling to enhance the efficiency of the algorithm. This attempt to reduce the search space is due to the fact that in most optimization pro...

Cytosolic NADP-specific isocitrate dehydrogenase was isolated from leaves of Pisum sativum. The purified enzyme was obtained by ammonium sulfate fractionation, ion exchange, affinity, and gel filtration chromatography. The purification procedure yields greater than 50% of the total enzyme activity originally present in the crude extract. The enzyme has a native molecular weight of 90 kilodalton...

Dipeptidyl aminopeptidase activity was found in the culture medium of Bacteroides gingivalis 381. The enzyme, hydrolyzing glycylprolyl-4-methylcoumaryl-7-amide, was purified 750-fold from culture medium by ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DEAE Bio Gel A column chromatography. The molecular weight, determined by gel filtration, was approximately 160,000. The iso...

A novel acid proteinase (Tropiase) was isolated from Candida tropicalis IFO 0589 by DE52-cellulose, and DEAE-Cosmogel column chromatographies. The purified tropiase gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme preparation had a molecular weight of 23,900, isoelectric point of p...

www.aana.com/aanajournalonline Huge hydrocephalus is defined as a head circumference larger than the length of the child. We discuss the perioperative anesthetic management of a case of huge hydrocephalus during ventriculoperitoneal shunt placement, focusing primarily on the airway management. The patient was a 4-month-old with a midline supratentorial lesion causing obstructive hydrocephalus d...

The principal enzyme of human prostatic acid phosphatase [orthophosphoric monoester phosphohydrolase (acid optimum), EC 3.1.3.2], which had been highly purified by affinity chromatography, isoelectric focusing, and gel filtrations, was shown to be homogeneous at pH 5.0 by sedimentation equilibrium analysis. The amino acid composition was determined and the sedimentation coefficient of the nativ...

The problem of finding a global optimum of an unconstrained multimodal function has been the subject of intensive study in recent years, giving rise to valuable advances in solution methods. We examine this problem within the framework of adaptive memory programming (AMP), focusing particularly on AMP strategies that derive from an integration of Scatter Search and Tabu Search. Computational co...

We present the investigation of a biogeochemical marine ecosystem model used as part of the climate change research focusing on the enhanced carbon dioxid concentration in the atmosphere. Numerical parameter optimization has been performed to improve represention of observational data using data assimilation techniques. Several local minima were found but no global optimum could be identified. ...

An extracellular proteinase was purified from culture filtrates of Cryptococcus neoformans NHPY24 by DEAE ion-exchange chromatography and gelatin affinity column chromatography with azoalbumin as the substrate. The molecular mass of the purified enzyme was 43 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, its pH optimum was 7.0 to 8.0, and maximal activity was obtained at pH ...