background  isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...

Organ cultures of rat kidney produce a factor capable of stimulating erythropoiesis in exhypoxic polycythemic mice. This material appears to be renal erythropoietic factor or erythrogenin, since biologic activity was observed only after incubation of the culture media with rat serum. Production of this material was similar in cultures prepared from four different anatomic regions of the kidney,...

Punch biopsies of human skin were obtained 1 day after irradiation with two minimal-erythema doses (MED) from either a UVB light source or a Solar Simulator and incubated in organ culture for 72 h. Organ culture fluids obtained at 24, 48 and 72 h were analyzed for collagenolytic activity and for reactivity with antibodies to matrix metalloproteinase-1 (MMP-1; interstitial collagenase) and MMP-1...

Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods. Organ culture is a powerful technique for studying renal development. It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1...

Organoids mimic the architecture and functions of a small organ. Organoid culture technique has been rapidly accepted by all research communities during the past decade to study stem cells, organ development and function, and patient-specific diseases. A protocol for organoid culture of human and mouse prostate epithelial and cancer tissues has been reported. However, organoid culture of the co...

Mucormycosis is a rare cause of necrotizing fasciitis in immunocompromised patients. We report a young female, who developed rhizopus necrotizing fasciitis of caesarean wound. The lady died secondary to non-responding sepsis and irreversible multi-organ failure. High index of suspicion can lead to early diagnosis by frozen section of histopathology and fungal culture technique. Aggressive surgi...

Peptococcus magnus is a biochemically inactive anaerobic Gram-positive coccus similar in morphology to the staphylococcus. It has been found (Smith, 1975) as part of the normal flora of the mouth, vagina, and skin. Previous reports (Pien et al., 1972; Spaulding et al., 1974) have described significant infections with this organism, but knowledge of its pathogenicity is incomplete since speciati...

Peptococcus magnus is a biochemically inactive anaerobic Gram-positive coccus similar in morphology to the staphylococcus. It has been found (Smith, 1975) as part of the normal flora of the mouth, vagina, and skin. Previous reports (Pien et al., 1972; Spaulding et al., 1974) have described significant infections with this organism, but knowledge of its pathogenicity is incomplete since speciati...

After endothelial damage in vivo, there is an induction of nitric oxide synthase (NOS) in the underlying smooth muscle cells. We hypothesized that intrinsic factors could induce NOS independently of blood elements. This was tested using an in vitro organ culture technique. Rat aortas with endothelium removed before 24-h organ culture (ERB) failed to constrict to phenylephrine after culture, whe...