Leucine oxidation and incorporation into protein were studied in cultured skeletal muscle cells to develop methods for measurement of absolute rates of these processes and to define the functional compartmentation of leucine within the muscle cell. The composition of the oxidation precursor pool and absolute rates of oxidation were determined from measurement of production of "CO2 from external...

This study was designed to evaluate the effects of dietary treatment, packaging, and irradiation singly or in combination on the oxidative stability of broiler chicken thigh meat. A total of 120 four-week-old chickens were divided into 12 pens (10 birds/pen), and 4 pens of broilers were randomly assigned to a control oxidized diet (5% oxidized oil) or an antioxidant-added diet [500 IU of vitami...

Reactive oxygen species (ROS) are not only a cause of oxidative stress in a range of disease conditions but are also important regulators of physiological pathways in vivo. One mechanism whereby ROS can regulate cell function is by modification of proteins through the reversible oxidation of their thiol groups. An experimental challenge has been the relative lack of techniques to probe the biol...

In this work, the effect of finishing diet (pasture- or mixed-diet) on lipid and protein oxidation in beef homogenates was evaluated. Oxidation was chemically induced by ferrous iron and hydrogen peroxide. Lipid and protein oxidation were respectively measured by determining TBA reactive substances (TBA-RS) and protein carbonyl groups (DNPH coupling method). Evaluation of the antioxidant status...